Skip to main content

Is there an association between vitamin D deficiency and adenotonsillar hypertrophy in children with sleep-disordered breathing?



Low vitamin D levels have been linked to the risk of sleep-disordered breathing (SDB) in children. Although adenotonsillar hypertrophy (ATH) is the major contributor to childhood SDB, the relationship between ATH and serum vitamin D is uncertain. We therefore investigated the relationship between vitamin D levels and associated factors in children with ATH.


We reviewed data from all children with SDB symptoms who were treated from December 2013 to February 2014. Of these, 88 children whose serum vitamin D levels were measured were enrolled in the study. We divided the children into four groups based on adenoidal and/or tonsillar hypertrophy. We conducted a retrospective chart review to analyze demographic data, the sizes of tonsils and adenoids, serum 25-hydroxy-vitamin D [25(OH)D] level, body mass index (BMI), and allergen sensitization patterns.


Children in the ATH group had a lower mean 25(OH)D level than did those in the control group (p < 0.05). Children with vitamin D deficiencies exhibited markedly higher frequencies of adenoidal and/or tonsillar hypertrophy than did those with sufficient vitamin D (p < 0.05). Spearman’s correlation analysis identified an inverse correlation between serum 25(OH)D levels and age, tonsil and adenoid size, and height (all p < 0.05). In a multiple regression analysis, tonsil and adenoid size as well as BMI-z score, were associated with 25(OH)D levels after controlling for age, sex, height, and mite sensitization (p < 0.05).


Our results suggest that low vitamin D levels are linked to ATH. Both the sizes of the adenoids and tonsils and the BMI-z score were associated with the 25(OH)D level. Therefore, measurement of the serum 25(OH)D level should be considered in children with ATH and SDB symptoms.

Peer Review reports


The spectrum of sleep-disordered breathing (SDB) is characterized by snoring, mouth-breathing, and pauses in breathing. SDB includes primary snoring, upper airway resistance syndrome, obstructive sleep apnea (OSA), and obstructive hypoventilation. Children with SDB not only experience sleep disturbances, but also neurocognitive impairment and attention problems. Adenotonsillar hypertrophy (ATH), the primary cause of OSA, is a common childhood disease that can be surgically treated [1,2,3,4].

Vitamin D, a fat-soluble vitamin, is synthesized in the skin upon exposure to sunlight and is also obtained from foods. Low vitamin D levels have been linked to many risk factors, including obesity, limited exposure to sunlight, prematurity, malabsorption, darkly pigmented skin, aging, chronic use of steroids or anticonvulsants, and low socioeconomic status [5,6,7]. In addition, several studies have reported that vitamin D deficiency may increase the risk of numerous acute/chronic otorhinolaryngologic conditions, including allergic rhinitis, chronic rhinosinusitis with nasal polyps, recurrent otitis media, acute respiratory infections, asthma, and benign paroxysmal positional vertigo [8,9,10,11,12,13].

Chronically low vitamin D levels may also be associated with sleep disorders [14, 15]. Recent studies reported that low vitamin D levels were related to OSA, and that continuous positive airway pressure treatment increased vitamin D levels in adults with OSA [16, 17]. Vitamin D deficiency has been linked to increases in the sizes of the tonsils and/or adenoids and thus to OSA development [18,19,20]. A decrease in vitamin D levels after an inflammatory insult has also been reported [21, 22], as has an association of low vitamin D levels and adenotonsillar diseases [23, 24]. In contrast, other studies found no association between serum vitamin D levels and such diseases [25, 26]. As the principal cause of vitamin D deficiency is inadequate exposure to sunlight, these conflicting results may be explained by differences in latitude and seasonal variations among studies. In addition, differences in ethnicity and skin color may also be in play [27,28,29].

In the present study, all subjects lived at the same latitude, were of the same ethnic group, and were evaluated only during winter, therefore reducing potential variations attributable to differences in the abovementioned factors. Our aim was to measure vitamin D levels and analyze associated factors in children with SDB.



We conducted a retrospective cross-sectional study at a single, university-based, secondary referral hospital. We recruited all children with SDB symptoms (e.g., snoring, mouth- breathing, paused breathing, and excessive daytime sleepiness) who were treated from December 2013 to February 2014.

In 2012, the authors established critical pathways for the clinical management of SDB, which state that the work up for SDB includes a physical examination, lateral plain X-ray of the nasopharynx, a quality of life evaluation using the Korean version [30] of the obstructive sleep apnea (KOSA)-18 survey [31], allergy evaluation, and measurement of the serum vitamin D level at our outpatient clinic.

The inclusion criteria of the present study were: (1) age 4–12 years; (2) habitual snoring, observed apnea, and/or mouth- breathing during sleep at least 1 year in duration; (3) total KOSA-18 score ≥ 60 (4) evaluation of atopic status using the multiple allergen simultaneous test (MAST); and (5) 25-hydroxy-vitamin D [25(OH)D] level measurement. The exclusion criteria were: (1) any craniofacial anomaly; (2) any anatomical abnormality, including nasal septal deviation, turbinate hypertrophy, and/or nasal polyps; (3) a recent history of nasal or upper airway infection; (4) malnutrition; (5) the use of vitamin D supplements or multivitamin agents; (6) a history of adenoidectomy and/or tonsillectomy; and/or (7) the use of anti-inflammatory and/or anti-allergic drugs within 4 weeks prior to enrollment.

We retrieved demographic, height, body weight, body mass index (BMI), BMI z-score, tonsil and adenoid size, atopic status, and serum vitamin D level data from medical records. We analyzed retrospectively collected data without collecting blood samples by our research group. We described the methods for in vitro IgE sensitization testing and measurement of serum vitamin D levels to clarify how these measurements have been obtained.

BMI was the body weight (kg) divided by the height squared (m2). We used the Korean national 2007 growth charts to determine BMI z-scores.

Tonsillar hypertrophy (TH) was graded using the Brodsky scale [32], as follows: grade 0 (tonsils situated in the tonsillar fossa); grade 1 (tonsils just outside of the tonsillar fossa and occupying ≤25% of the airway); grade 2 (tonsils occupying 26–50% of the airway); grade 3 (tonsils occupying 51–75% of airway); and grade 4 (tonsils occupying > 75% of the airway). We used the adenoidal-nasopharyngeal ratio (ANR,) obtained from a lateral plain X-ray of the nasopharynx, to represent the adenoidal size. The depths of the adenoids and nasopharynx were measured using the standard landmarks of Fujioka [33]. The adenoids were measured by drawing lines perpendicular to lines drawn along the straight region of the anterior margin of the basiocciput to the point of maximal adenoidal convexity. The nasopharynx was measured by drawing a line from the anterior inferior edge of the sphenobasioccipital synchondrosis to the posterior superior margin of the hard palate. The ANR was then determined by dividing the first measurement by the second.

We defined grade 3 or 4 tonsils as TH. We defined an ANR ≥ 0.8 as indicative of adenoidal hypertrophy (AH). We then divided the children into four groups: control, AH, TH, and ATH.

The Korean version of the obstructive sleep apnea (KOSA)-18

To assess quality of life, caregivers completed the KOSA-18 questionnaire, a disease-specific questionnaire validated in Korea. The 18 items of the KOSA-18 are grouped within five domains (sleep disturbance, physical symptoms, emotional distress, daytime function, and caregiver concerns) and are scored using a 7-point ordinal scale, followed by summing of the scores. Possible scores range from 18 to 126 points, with a higher score indicating a worse quality of life. Franco et al. suggested a clinical classification based on the OSA-18, with scores < 60 suggesting a small impact on the health-related quality of life, scores between 60 and 80 a moderate impact, and scores > 80 a large impact [31]. According to this classification, we used the KOSA-18 as one of the inclusion criteria and children with total scores of ≥60 were included in this study.

Determination of serum 25-hydroxy-vitamin D levels

To evaluate vitamin D status, serum levels of 25-hydroxy-vitamin D (25(OH)D) were measured using a direct competitive chemiluminescence immunoassay (CLIA; LIAISON® 25 OH vitamin D assay; DiaSorin, Saluggia, Italy). The intra- and interassay coefficients of variation for 25(OH)D were 3–6 and 7–11%, respectively.

Sensitization patterns of the allergens

In vitro IgE sensitization testing was carried out using the multiple allergen simultaneous test (MAST) (RoboScreen™; Bee Robotics Ltd., Gwynedd, UK). The panel consists of 39 allergens, including foods, tree/grass/weed pollens, fungi, dogs, cats, cockroaches, and house dust mites. A score ≥ 2 was interpreted as positive [34].

Statistical analysis

Statistical analyses were performed using SPSS for Windows software (ver. 15.0; SPSS, Inc., Chicago, IL). Qualitative parameters were evaluated with a chi-square test, and quantitative parameters using a Kruskal-Wallis test. Factors associated with vitamin D deficiency were evaluated using Spearman’s correlation test. For multivariate analysis, a multiple regression analysis was used. All statistical tests were two-tailed. A P-value < 0.05 was considered to indicate statistical significance.

Ethics statement

Written informed consent was not obtained because of the retrospective nature of the study. However, the study protocol was approved by our Institutional Review Board (IRB policy NO. UC15RISI0035).


We included 88 patients [59 males (67.0%) and 29 females (33.0%)] of mean age 8.9 ± 2.5 years. The mean serum 25(OH)D level was 19.4 ± 5.1 ng/mL. A serum 25(OH)D level < 20 ng/mL was considered to reflect a vitamin D deficiency [35]; 52.3% of the children were deficient. The frequency of AH and/or TH in children with vitamin D deficiency and sufficiency was 91.3 and 71.4%, respectively. Deficient children exhibited markedly higher frequency rates of AH and/or TH than did those exhibiting vitamin sufficiency (p = 0.035, Fig. 1).

Fig. 1

Comparisons of frequencies of adenoid and/or tonsillar hypertrophy by serum 25(OH)D level. Vitamin D-deficient: 25(OH)D < 20 ng/mL; vitamin D-sufficient: 25(OH)D ≥ 20 ng/mL

Children with ATH had lower 25(OH)D levels

We compared the clinical characteristics of the control, AH, TH, and ATH groups. The numbers of children per group were as follows: control, 16 (18.2%); AH, 18 (20.4%); TH, 19 (21.6%), and ATH, 35 (39.8%). The children in the ATH group were younger than those in the AH group (p = 0.021). The ATH group had more females than the control and AH groups (p = 0.002 and 0.042, respectively). We found no significant difference in height, body weight, BMI, or BMI z-score among the four groups (Table 1). The mean serum 25(OH)D levels of the four groups were as follows: control, 22.5 ± 4.3; AH, 18.7 ± 6.5; TH, 19.4 ± 4.5; and ATH, 18.4 ± 4.5 ng/mL. The children in the ATH group had the lowest mean 25(OH)D level (i.e., lower than that of the control group [p = 0.01, Fig. 2]).

Table 1 Characteristics of 88 children with or without adenoid and/or tonsillar hypertrophy
Fig. 2

Serum 25(OH)D levels in children with or without adenoid and/or tonsillar hypertrophy. *: p < 0.05

Allergen sensitization

A comparison of the atopic status among the four groups showed that the mean number of sensitized allergens in the control, AH, TH, and ATH groups was 3.0, 2.3, 1.5, and 1.2, respectively. The mean was somewhat higher in the control group than in the other groups, but the difference was not significant. The prevalence of atopy in the control, AH, TH, and ATH groups was 50.0, 77.8, 68.4, and 42.9%, respectively. The higher prevalence of atopy in the AH group than in the other groups was also not statistically significant.

Negative association of age, tonsil size, ANR, and height with serum 25(OH)D

We used Spearman’s correlation test to explore correlations between the serum 25(OH)D level and other variables (Table 2). Age (r = − 0.26, p = 0.001), tonsil size (r = − 0.46, p = 0.002), ANR (r = − 0.40, p = 0.001), and height (r = − 0.33, p = 0.020) were negatively associated with the serum 25(OH)D level. Body weight, BMI, and BMI z-score also exhibited negative relationships, but these were not statistically significant.

Table 2 Correlation coefficients for serum 25(OH)D levels by Spearman’s rank correlation rho

Marked association of tonsil size and ANR with serum 25(OH)D

We used a multiple regression analysis to seek factors associated with vitamin D level (Table 3). In model 1, the serum 25(OH)D level was inversely associated with tonsil size (β = − 0.41, p = 0.001), ANR (β = − 0.21, p = 0.48), and BMI-z score (β = − 1.07, p = 0.029) after adjusting for age and sex. These relationships persisted even after further adjustment in model 2 (tonsil size, β = − 0.40, p = 0.001; ANR, β = − 0.22, p = 0.043; and BMI-z score, β = − 1.07, p = 0.001).

Table 3 Multiple regression models of serum 25(OH)D level


OSA is associated with an increased risk of vitamin D deficiency. Low vitamin D level increases the risk of OSA by promoting ATH, airway muscle myopathy, and/or chronic rhinitis [23, 36,37,38]. Recent studies in adults showed that a large proportion of those with OSA also had a vitamin D deficiency [39, 40]. ATH is the most common cause of OSA in children. However, data on the relationship between vitamin D deficiency and AH and/or TH are conflicting [23,24,25,26]. In the present study, we used only winter data from children of the same ethnicity (Korean) living at the same latitude (37° 76′ N) to control for contributions made by these factors to the extent of sunlight exposure. We found that the 25(OH)D level was reduced in children with ATH, AH, or TH. The sizes of the adenoids and tonsils, and BMI-z score predicted the serum 25(OH)D level.

We found that 52.3% of all children were vitamin D-deficient. In a nationwide Korean cross-sectional survey, the prevalence of vitamin D deficiency in randomly selected children was 18.4%, thus lower than that in our study. However, the cited survey was conducted in autumn [41]. Another Korean study, conducted in autumn, winter, and spring, found that 59.1% of all children were vitamin D-deficient [42]. These among-study differences are attributable to seasonal variations, participant age, and the prevalence of underlying conditions.

We found that the sizes of the tonsils and adenoids were negatively associated with the serum 25(OH)D level. Several studies have reported relationships between low vitamin D levels and adenotonsillar diseases [23, 24]. A Turkish study found that children with recurrent tonsillitis and allergic rhinitis had significantly lower 1,25-dihydroxyvitamin D [1,25(OH)2D] levels than controls [24]. However, it was not clear that the low vitamin D levels were caused by the tonsillitis or allergic rhinitis, and the seasons in which blood samples were collected were not considered. A pilot study performed in the US found no difference in the vitamin D levels of children undergoing adenotonsillectomies and controls. However, the study included children who underwent adenotonsillectomies not only because of obstruction but also to treat recurrent infections. Again, the seasons in which blood was collected were not reported [25]. As mentioned above, these conflicting results may be explained by differences in latitude, season, ethnicity, and skin pigmentation [6, 29].

Vitamin D deficiency may increase ATH via inadequate regulation of the immune system. Vitamin D receptors are found on T cells, B cells, antigen-presenting cells, macrophages, and dendritic cells. Vitamin D immunomodulates both innate and adaptive immune responses [18, 43]. In terms of the innate immune system, vitamin D increases the production of antimicrobial peptides, including defensin ß and cathelicidin [44, 45]. In the adaptive immune system, the vitamin D inhibits the proliferation of activated lymphocytes, reduces the production of inflammatory cytokines, and promotes the development of induced regulatory T cells [46,47,48]. Vitamin D deficiency increases the risk of upper and lower airway infections [49, 50]. Many studies have shown that low vitamin D levels are associated with respiratory tract infections and that vitamin D supplements exert beneficial effects during the treatment of infectious diseases [51, 52], although some randomized controlled trials found that vitamin D afforded no benefit in those treated for infectious diseases [53,54,55]. A recent systematic review and meta-analysis reported that vitamin D supplements had a protective effect against acute respiratory infection, particularly in patients with profound vitamin D deficiency [12]. In terms of the effects of the vitamin D on the adenoids and tonsils, a deficiency may increase recurrent infections. In addition, vitamin D regulates human tonsillar T cells and a deficiency may trigger TH [18, 56]. Interestingly, recent studies suggested that low vitamin D levels are the result rather than the cause of the inflammatory process, as bacterial infection may induce the intracellular conversion of 25(OH)D to 1,25(OH)2D, resulting in high 1,25(OH)2D and low 25(OH)D [57,58,59]. Therefore, the low vitamin D levels in ATH patients may be a consequence of recurrent adenotonsillitis by bacterial infections.

Many studies have found that increased BMI is associated with vitamin D insufficiency in children [60, 61]. Holick et al. [35] reported that the bioavailability of vitamin D in obesity was reduced because the vitamin was deposited in the body fat. The 2003–2006 USA National Health and Nutrition Examination Survey (which assessed children and adolescents) found that vitamin D deficiency was very prevalent in overweight and obese children [62]. A study of Korean children also revealed that the 25(OH)D level was lower in an overweight compared to a normal-weight group [63]. Consistent with the results of these previous studies, we found that the BMI-z score was negatively associated with the serum 25(OH)D level.

In terms of allergen sensitization, we found no significant difference in either the numbers of allergens to which children were sensitized or the prevalence of atopy among the four groups. Two explanations are possible. One is that the sensitivity of the MAST is low. The other is that children with both allergic rhinitis and turbinate hypertrophy were excluded. Thus, not all children with allergic rhinitis were included. Many studies have found that low vitamin D levels are associated with childhood allergic diseases, including allergic rhinitis, asthma, and atopic dermatitis [64, 65]. A recent Australian study found that a low vitamin D level in early childhood was associated with an increased risk of asthma and early allergic sensitization [65]. In Korea, a recent study showed that low vitamin D levels were associated with symptoms of allergic rhinitis and atopic dermatitis [41]. However, some studies yielded different results [66, 67]. A study of two large birth cohorts found that vitamin D had no protective effect against asthma or allergic rhinitis, and was positively associated with eczema, in 10-year-old children [66]. Thus, no conclusive association has been demonstrated between vitamin D and allergic disease.

A strength of our study is that it was conducted during one season in children of the same ethnicity and living at the same latitude. We thus controlled for several possible confounders. Second, we evaluated allergen sensitization patterns; other similar studies did not [20, 25]. Many studies have reported associations between vitamin D levels and allergic diseases [68, 69]; an evaluation of atopic status is essential when studying the effects of variations in vitamin D levels. Third, we defined clinical features predictive of vitamin D deficiency. Physicians can easily measure the sizes of the tonsils and adenoids, body weight, and BMI in children with SDB.

However, there are some limitations to our study. First, the sample size was too small to allow detailed generalizations to be made. Second, we did not use polysomnography (PSG) for the evaluation of SDB. However, although PSG is the gold standard for the diagnosis of SDB, in practice, the test is time-consuming and cannot be easily performed in all patients. A study in the USA showed that only 10% of children who underwent adenotonsillectomy also underwent a PSG evaluation [70]. In addition, Franco et al. reported that OSA-18 scores correlated significantly with the respiratory distress index determined by PSG [31]. We used the KOSA-18 score [30] as one of the inclusion criteria in our study and included children whose health-related quality of life was moderately to severely affected by OSA. Third, we used the MAST rather than the skin prick test (SPT). However, although the SPT remains a major diagnostic tool, the MAST has the advantage that many allergens can be tested simultaneously. Also, MAST data correlate well with those of the SPT in rhinitis patients, which suggests that the MAST can serve as an alternative to the SPT [71]. Finally, we performed only a retrospective chart review. Additional, larger studies incorporating polysomnographic data may be required before general conclusions can be drawn.


Approximately half of all children with SDB were vitamin D-deficient. The sizes of the adenoids and tonsils, and BMI-z score were negatively associated with the serum 25(OH)D level. Our results suggest that SDB children with vitamin D deficiencies may need to be evaluated in terms of AH and/or TH, and vice versa.





Serum 25-hydroxy-vitamin D


Adenoidal hypertrophy


Adenoidal-nasopharyngeal ratio


Adenotonsillar hypertrophy


Body mass index

KOSA-18 survey:

Korean version of the obstructive sleep apnea-18 survey


Multiple allergen simultaneous test


Obstructive sleep apnea




Sleep-disordered breathing


Skin prick test


Tonsillar hypertrophy


  1. 1.

    Kobayashi R, Miyazaki S, Karaki M, Hoshikawa H, Nakata S, Hara H, et al. Obstructive sleep apnea in Asian primary school children. Sleep Breath. 2014;18:483–9.

    Article  PubMed  Google Scholar 

  2. 2.

    Kaditis AG, Alonso Alvarez ML, Boudewyns A, Alexopoulos EI, Ersu R, Joosten K, et al. Obstructive sleep disordered breathing in 2- to 18-year-old children: diagnosis and management. Eur Respir J. 2016;47:69–94.

    Article  PubMed  Google Scholar 

  3. 3.

    Sedky K, Bennett DS, Carvalho KS. Attention deficit hyperactivity disorder and sleep disordered breathing in pediatric populations: a meta-analysis. Sleep Med Rev. 2014;18:349–56.

    Article  PubMed  Google Scholar 

  4. 4.

    Waters KA, Chawla J, Harris MA, Dakin C, Heussler H, Black R, et al. Rationale for and design of the “POSTA” study: evaluation of neurocognitive outcomes after immediate adenotonsillectomy compared to watchful waiting in preschool children. BMC Pediatr. 2017;17:47.

    Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Bouillon R. Comparative analysis of nutritional guidelines for vitamin D. Nat Rev Endocrinol. 2017;13:466–79.

    Article  PubMed  CAS  Google Scholar 

  6. 6.

    Wacker M, Holick MF. Sunlight and Vitamin D: a global perspective for health. Dermatoendocrinol. 2013;5:51–108.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. 7.

    Ariganjoye R. Pediatric Hypovitaminosis D: molecular perspectives and clinical implications. Glob Pediatr Health. 2017;

  8. 8.

    Akbar NA, Zacharek MA. Vitamin D: immunomodulation of asthma, allergic rhinitis, and chronic rhinosinusitis. Curr Opin Otolaryngol Head Neck Surg. 2011;19:224–8.

    Article  PubMed  Google Scholar 

  9. 9.

    Carroll WW, Schlosser RJ, O'Connell BP, Soler ZM, Mulligan JK. Vitamin D deficiency is associated with increased human sinonasal fibroblast proliferation in chronic rhinosinusitis with nasal polyps. Int Forum Allergy Rhinol. 2016;6:605–10.

    Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Walker RE, Bartley J, Camargo CA Jr, Flint D, Thompson JMD, Mitchell EA. Higher serum 25(OH)D concentration is associated with lower risk of chronic otitis media with effusion: a case-control study. Acta Paediatr. 2017;106(9):1487–92.

    Article  PubMed  CAS  Google Scholar 

  11. 11.

    Lee SB, Lee CH, Kim YJ, Kim HM. Biochemical markers of bone turnover in benign paroxysmal positional vertigo. PLoS One. 2017;12:e0176011.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  12. 12.

    Martineau AR, Jolliffe DA, Hooper RL, Greenberg L, Aloia JF, Bergman P, et al. Vitamin D supplementation to prevent acute respiratory tract infections: systematic review and meta-analysis of individual participant data. BMJ. 2017;356:i6583.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  13. 13.

    Martineau AR, Cates CJ, Urashima M, Jensen M, Griffiths AP, Nurmatov U, et al. Vitamin D for the management of asthma. Cochrane Database Syst Rev. 2016;9:Cd011511.

    PubMed  Google Scholar 

  14. 14.

    Ozgurhan G, Vehapoglu A, Vermezoglu O, Temiz RN, Guney A, Hacihamdioglu B. Risk assessment of obstructive sleep apnea syndrome in pediatric patients with vitamin D deficiency: a questionnaire-based study. Medicine (Baltimore). 2016;95:e4632.

    Article  CAS  Google Scholar 

  15. 15.

    Zicari AM, Occasi F, Di Mauro F, Lollobrigida V, Di Fraia M, Savastano V, et al. Mean platelet volume, vitamin D and C reactive protein levels in normal weight children with primary snoring and obstructive sleep apnea syndrome. PLoS One. 2016;11(4):e0152497.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  16. 16.

    Liguori C, Izzi F, Mercuri NB, Romigi A, Cordella A, Tarantino U, et al. Vitamin D status of male OSAS patients improved after long-term CPAP treatment mainly in obese subjects. Sleep Med. 2017;29:81–5.

    Article  PubMed  Google Scholar 

  17. 17.

    Liguori C, Romigi A, Izzi F, Mercuri NB, Cordella A, Tarquini E, et al. Continuous positive airway pressure treatment increases serum vitamin D levels in male patients with obstructive sleep apnea. J Clin Sleep Med. 2015;11(6):603–7.

    PubMed  PubMed Central  Google Scholar 

  18. 18.

    McCarty DE, Chesson AL Jr, Jain SK, Marino AA. The link between vitamin D metabolism and sleep medicine. Sleep Med Rev. 2014;18:311–9.

    Article  PubMed  Google Scholar 

  19. 19.

    Bozkurt NC, Cakal E, Sahin M, Ozkaya EC, Firat H, Delibasi T. The relation of serum 25-hydroxyvitamin-D levels with severity of obstructive sleep apnea and glucose metabolism abnormalities. Endocrine. 2012;41:518–25.

    Article  PubMed  CAS  Google Scholar 

  20. 20.

    Kheirandish-Gozal L, Peris E, Gozal D. Vitamin D levels and obstructive sleep apnoea in children. Sleep Med. 2014;15:459–63.

    Article  PubMed  PubMed Central  Google Scholar 

  21. 21.

    Reid D, Toole BJ, Knox S, Talwar D, Harten J, O'Reilly DS, et al. The relation between acute changes in the systemic inflammatory response and plasma 25-hydroxyvitamin D concentrations after elective knee arthroplasty. Am J Clin Nutr. 2011;93(5):1006–11.

    Article  PubMed  CAS  Google Scholar 

  22. 22.

    Henriksen VT, Rogers VE, Rasmussen GL, Trawick RH, Momberger NG, Aguirre D, et al. Pro-inflammatory cytokines mediate the decrease in serum 25(OH)D concentrations after total knee arthroplasty? Med Hypotheses. 2014;82(2):134–7.

    Article  PubMed  CAS  Google Scholar 

  23. 23.

    Reid D, Morton R, Salkeld L, Bartley J. Vitamin D and tonsil disease--preliminary observations. Int J Pediatr Otorhinolaryngol. 2011;75:261–4.

    Article  PubMed  Google Scholar 

  24. 24.

    San T, Muluk NB, Cingi C. 1,25(OH)(2)D-3 and specific IgE levels in children with recurrent tonsillitis, and allergic rhinitis. Int J Pediatr Otorhinolaryngol. 2013;77:1506–11.

    Article  PubMed  Google Scholar 

  25. 25.

    Esteitie R, Naclerio RM, Baroody FM. Vitamin D levels in children undergoing adenotonsillectomies. Int J Pediatr Otorhinolaryngol. 2010;74:1075–7.

    Article  PubMed  Google Scholar 

  26. 26.

    Aydın S, Aslan I, Yıldız I, Ağaçhan B, Toptaş B, Toprak S, et al. Vitamin D levels in children with recurrent tonsillitis. Int J Pediatr Otorhinolaryngol. 2011;75:364–7.

    Article  PubMed  Google Scholar 

  27. 27.

    Schramm S, Lahner H, Jöckel KH, Erbel R, Führer D, Moebus S, et al. Impact of season and different vitamin D thresholds on prevalence of vitamin D deficiency in epidemiological cohorts-a note of caution. Endocrine. 2017;56:658–66.

    Article  PubMed  CAS  Google Scholar 

  28. 28.

    Fuleihan Gel H, Bouillon R, Clarke B, Chakhtoura M, Cooper C, McClung M, et al. Serum 25-Hydroxyvitamin D levels: variability, knowledge gaps, and the concept of a desirable range. J Bone Miner Res. 2015;30:1119–33.

    Article  CAS  Google Scholar 

  29. 29.

    Mazahery H, von Hurst PR. Factors affecting 25-Hydroxyvitamin D concentration in response to vitamin D supplementation. Nutrients. 2015;7:5111–42.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  30. 30.

    Park CS, Guilleminault C, Hwang SH, Jeong JH, Park DS, Maeng JH. Correlation of salivary cortisol level with obstructive sleep apnea syndrome in pediatric subjects. Sleep Med. 2013;14(10):978–84.

    Article  PubMed  Google Scholar 

  31. 31.

    Franco RA Jr, Rosenfeld RM, Rao M. First place--resident clinical science award 1999. Quality of life for children with obstructive sleep apnea. Otolaryngol Head Neck Surg. 2000;123:9–16.

    Article  PubMed  Google Scholar 

  32. 32.

    Brodsky L, Moore L, Stanievich JF. A comparison of tonsillar size and oropharyngeal dimensions in children with obstructive adenotonsillar hypertrophy. Int J Pediatr Otorhinolaryngol. 1987;13:149–56.

    Article  PubMed  CAS  Google Scholar 

  33. 33.

    Fujioka M, Young LW, Girdany BR. Radiographic evaluation of adenoidal size in children: adenoidal-nasopharyngeal ratio. AJR Am J Roentgenol. 1979;133:401–4.

    Article  PubMed  CAS  Google Scholar 

  34. 34.

    Nepper-Christensen S, Backer V, DuBuske LM, Nolte H. In vitro diagnostic evaluation of patients with inhalant allergies: summary of probability outcomes comparing results of CLA- and CAP-specific immunoglobulin E test systems. Allergy Asthma Proc. 2003;24:253–8.

    PubMed  CAS  Google Scholar 

  35. 35.

    Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr. 2008;87:1080S–6S.

    Article  PubMed  CAS  Google Scholar 

  36. 36.

    Atan Sahin O, Kececioglu N, Serdar M, Ozpinar A. The association of residential mold exposure and adenotonsillar hypertrophy in children living in damp environments. Int J Pediatr Otorhinolaryngol. 2016;88:233–8.

    Article  PubMed  Google Scholar 

  37. 37.

    Dogru M, Suleyman A. Serum 25-hydroxyvitamin D3 levels in children with allergic or nonallergic rhinitis. Int J Pediatr Otorhinolaryngol. 2016;80:39–42.

    Article  PubMed  Google Scholar 

  38. 38.

    Prabhala A, Garg R, Dandona P. Severe myopathy associated with vitamin D deficiency in western New York. Arch Intern Med. 2000;160(8):1199–203.

    Article  PubMed  CAS  Google Scholar 

  39. 39.

    Piovezan RD, Hirotsu C, Feres MC, Cintra FD, Andersen ML, Tufik S, et al. Obstructive sleep apnea and objective short sleep duration are independently associated with the risk of serum vitamin D deficiency. PLoS One. 2017;12(7):e0180901.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  40. 40.

    Salepci B, Caglayan B, Nahid P, Parmaksiz ET, Kiral N, Fidan A, et al. Vitamin D deficiency in patients referred for evaluation of obstructive sleep apnea. J Clin Sleep Med. 2017;13(4):607–12.

    Article  PubMed  PubMed Central  Google Scholar 

  41. 41.

    Yang HK, Choi J, Kim WK, Lee SY, Park YM, Han MY, et al. The association between hypovitaminosis D and pediatric allergic diseases: a Korean nationwide population-based study. Allergy Asthma Proc. 2016;37:64–9.

    Article  PubMed  Google Scholar 

  42. 42.

    Roh YE, Kim BR, Choi WB, Kim YM, Cho MJ, Kim HY, et al. Vitamin D deficiency in children aged 6 to 12 years: single center's experience in Busan. Ann Pediatr Endocrinol Metab. 2016;21:149–54.

    Article  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Rosendahl J, Holmlund-Suila E, Helve O, Viljakainen H, Hauta-Alus H, Valkama S, et al. 25-hydroxyvitamin D correlates with inflammatory markers in cord blood of healthy newborns. Pediatr Res. 2017;81(5):731–5.

    Article  PubMed  CAS  Google Scholar 

  44. 44.

    Liu PT, Stenger S, Li H, Wenzel L, Tan BH, Krutzik SR, et al. Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science. 2006;311(5768):1770–3.

    Article  PubMed  CAS  Google Scholar 

  45. 45.

    Wang TT, Nestel FP, Bourdeau V, Nagai Y, Wang Q, Liao J, et al. Cutting edge: 1,25-dihydroxyvitamin D3 is a direct inducer of antimicrobial peptide gene expression. J Immunol. 2004;173(5):2909–12.

    Article  PubMed  CAS  Google Scholar 

  46. 46.

    Xie Z, Chen J, Zheng C, Wu J, Cheng Y, Zhu S, et al. 1,25-dihydroxyvitamin D-induced dendritic cells suppress experimental autoimmune encephalomyelitis by increasing proportions of the regulatory lymphocytes and reducing T helper type 1 and type 17 cells. Immunology. 2017;152(3):414–24.

    Article  PubMed  CAS  Google Scholar 

  47. 47.

    Mansouri L, Lundwall K, Moshfegh A, Jacobson SH, Lundahl J, Spaak J. Vitamin D receptor activation reduces inflammatory cytokines and plasma MicroRNAs in moderate chronic kidney disease - a randomized trial. BMC Nephrol. 2017;18(1):161.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  48. 48.

    Zhou Q, Qin S, Zhang J, Zhon L, Pen Z, Xing T. 1,25(OH)2D3 induces regulatory T cell differentiation by influencing the VDR/PLC-γ1/TGF-β1/pathway. Mol Immunol. 2017;91:156–64.

    Article  PubMed  CAS  Google Scholar 

  49. 49.

    Ginde AA, Mansbach JM, Camargo CA Jr. Vitamin D, respiratory infections, and asthma. Curr Allergy Asthma Rep. 2009;9:81–7.

    Article  PubMed  CAS  Google Scholar 

  50. 50.

    Mora JR, Iwata M, von Andrian UH. Vitamin effects on the immune system: vitamins a and D take Centre stage. Nat Rev Immunol. 2008;8:685–98.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  51. 51.

    Ginde AA, Mansbach JM, Camargo CA Jr. Association between serum 25-hydroxyvitamin D level and upper respiratory tract infection in the third National Health and nutrition examination survey. Arch Intern Med. 2009;169:384–90.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  52. 52.

    Ginde AA, Blatchford P, Breese K, Zarrabi L, Linnebur SA, Wallace JI, et al. High-dose monthly vitamin D for prevention of acute respiratory infection in older long-term care residents: a randomized clinical trial. J Am Geriatr Soc. 2017;65(3):496–503.

    Article  PubMed  Google Scholar 

  53. 53.

    Rees JR, Hendricks K, Barry EL, Peacock JL, Mott LA, Sandler RS, et al. Vitamin D3 supplementation and upper respiratory tract infections in a randomized, controlled trial. Clin Infect Dis. 2013;57(10):1384–92.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  54. 54.

    Li-Ng M, Aloia JF, Pollack S, Cunha BA, Mikhail M, Yeh J, et al. A randomized controlled trial of vitamin D3 supplementation for the prevention of symptomatic upper respiratory tract infections. Epidemiol Infect. 2009;137:1396–404.

    Article  PubMed  CAS  Google Scholar 

  55. 55.

    Somnath SH, Biswal N, Chandrasekaran V, Jagadisan B, Bobby Z. Therapeutic effect of vitamin D in acute lower respiratory infection: a randomized controlled trial. Clin Nutr ESPEN. 2017;20:24–8.

    Article  PubMed  Google Scholar 

  56. 56.

    Nunn JD, Katz DR, Barker S, et al. Regulation of human tonsillar T-cell proliferation by the active metabolite of vitamin D3. Immunology. 1986;59:479–84.

    PubMed  PubMed Central  CAS  Google Scholar 

  57. 57.

    Mangin M, Sinha R, Fincher K. Inflammation and vitamin D: the infection connection. Inflamm Res. 2014;63(10):803–19.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  58. 58.

    Waldron JL, Ashby HL, Cornes MP, Bechervaise J, Razavi C, Thomas OL, et al. Vitamin D: a negative acute phase reactant. J Clin Pathol. 2013;66(7):620–2.

    Article  PubMed  CAS  Google Scholar 

  59. 59.

    Custódio G, Schwarz P, Crispim D, Moraes RB, Czepielewski M, Leitão CB, et al. Association between vitamin D levels and inflammatory activity in brain death: a prospective study. Transpl Immunol. 2018; Epub ahead of print

  60. 60.

    Szlagatys-Sidorkiewicz A, Brzezinski M, Jankowska A, Metelska P, Slominska-Fraczek M, Socha P. Long-term effects of vitamin D supplementation in vitamin D deficient obese children participating in an integrated weight-loss programme (a double-blind placebo-controlled study) - rationale for the study design. BMC Pediatr. 2017;17:97.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  61. 61.

    Barja-Fernández S, Aguilera CM, Martínez-Silva I, Vazquez R, Gil-Campos M, Olza J, et al. 25-Hydroxyvitamin D levels of children are inversely related to adiposity assessed by body mass index. J Physiol Biochem. 2018;74(1):111–8.

    Article  PubMed  CAS  Google Scholar 

  62. 62.

    Turer CB, Lin H, Flores G. Prevalence of vitamin D deficiency among overweight and obese US children. Pediatrics. 2013;131:e152–61.

    Article  PubMed  Google Scholar 

  63. 63.

    Chung IH, Kim HJ, Chung S, Yoo EG. Vitamin D deficiency in Korean children: prevalence, risk factors, and the relationship with parathyroid hormone levels. Ann Pediatr Endocrinol Metab. 2014;19:86–90.

    Article  PubMed  PubMed Central  Google Scholar 

  64. 64.

    Aryan Z, Rezaei N, Camargo CA Jr. Vitamin D status, aeroallergen sensitization, and allergic rhinitis: a systematic review and meta-analysis. Int Rev Immunol. 2017;36:41–53.

    Article  PubMed  CAS  Google Scholar 

  65. 65.

    Hollams EM, Teo SM, Kusel M, et al. Vitamin D over the first decade and susceptibility to childhood allergy and asthma. J Allergy Clin Immunol. 2017;139:472–81.

    Article  PubMed  CAS  Google Scholar 

  66. 66.

    Wawro N, Heinrich J, Thiering E, Kratzsch J, Schaaf B, Hoffmann B, et al. Serum 25(OH)D concentrations and atopic diseases at age 10: results from the GINIplus and LISAplus birth cohort studies. BMC Pediatr. 2014;14:286.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  67. 67.

    Cairncross C, Grant C, Stonehouse W, Conlon C, McDonald B, Houghton L, et al. The relationship between vitamin D status and allergic diseases in New Zealand preschool children. Nutrients. 2016;8:6.

    Article  CAS  Google Scholar 

  68. 68.

    Arikoglu T, Kuyucu S, Karaismailoglu E, Batmaz SB, Balci S. The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children. Allergy Asthma Proc. 2015;36(4):51–8.

    Article  PubMed  Google Scholar 

  69. 69.

    Chiu CY, Su KW, Tsai MH, Hua MC, Liao SL, Lai SH, et al. Longitudinal vitamin D deficiency is inversely related to mite sensitization in early childhood. Pediatr Allergy Immunol. 2017; Epub ahead of print

  70. 70.

    Mitchell RB, Pereira KD, Friedman NR. Sleep-disordered breathing in children: survey of current practice. Laryngoscope. 2006;116:956–8.

    Article  PubMed  Google Scholar 

  71. 71.

    Cho JH, Suh JD, Kim JK, Hong SC, Park IH, Lee HM. Correlation between skin-prick testing, individual specific IgE tests, and a multiallergen IgE assay for allergy detection in patients with chronic rhinitis. Am J Rhinol Allergy. 2014;28:388–91.

    Article  PubMed  Google Scholar 

Download references


We thank Dr. Daeyoung Roh for contributing to the statistical analysis.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Author information




JHS and BGK conceived and designed the study. JHS, BYK and HK contributed to acquisition of the data. JHS, BYK, SWhK and SWoK analyzed and interpreted the data. JHS drafted and revised the manuscript. All authors involved in drafting the manuscript or revising it and approved the final manuscript.

Corresponding author

Correspondence to Ji-Hyeon Shin.

Ethics declarations

Ethics approval and consent to participate

The study protocol was approved by the Institutional Review Board of Uijeongbu St. Mary’s Hospital (IRB policy No. UC15RISI0035). Since this study is a retrospective chart review study the need for written consent was formally waved by the IRB of Uijeongbu St. Mary’s Hospital.

Consent for publication

Not applicable

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Shin, J., Kim, B., Kim, B.Y. et al. Is there an association between vitamin D deficiency and adenotonsillar hypertrophy in children with sleep-disordered breathing?. BMC Pediatr 18, 196 (2018).

Download citation


  • Vitamin D
  • Adenoids
  • Tonsils
  • Sleep-disordered breathing
  • Body mass index
  • Child