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Oxidative stress in term small for gestational age neonates born to undernourished mothers: a case control study
© Gupta et al; licensee BioMed Central Ltd. 2004
Received: 12 February 2004
Accepted: 20 July 2004
Published: 20 July 2004
The objective of this study was to assess the status of oxidative stress in term small for gestational age (SGA) newborn infants born to undernourished mothers by estimating levels of erythrocyte superoxide dismutase (SOD), catalase, reduced glutathione, and serum malondialdehyde (MDA) in cord blood and comparing them to healthy appropriate for gestational age (AGA) controls. This was done in a case control design at a tertiary level teaching hospital.
We included 20 singleton healthy SGA newborn infants born between 38–40 weeks to undernourished mothers with a) post-pregnancy weight < 50 kg or height < 145 cm AND b) hemoglobin < 8.0 g/dL or serum albumin < 2.5 g/dL. An equal number of age and sex matched AGA newborn infants born to healthy mothers served as Controls. Mothers with other risk factors and newborns with complications during delivery or immediate newborn period were excluded. MDA, SOD, catalase and reduced glutathione were measured in the cord blood of all neonates and compared between the groups (unpaired t test); levels were also correlated to maternal weight, height, hemoglobin, and albumin by both univariate (pearsonian correlation) and multivariate (multiple regression) analysis.
The activity of MDA was increased (5.33 ± 0.72 vs 2.55 ± 0.22 nmol/mL; P < 0.0001) while levels of superoxide dismutase (493.6 ± 54.9 vs. 786.8 ± 79.1 U/g Hb; P < 0.0001), catalase (1.48 ± 0.24 vs. 2.31 ± 0.20 U/g Hb; P < 0.0001) and reduced glutathione (2.84 ± 0.37 vs 6.42 ± 0.23 Umol/g Hb, P < 0.0001) were decreased in term SGA born to undernourished mothers as compared to term AGA born to healthy mothers. On univariate analysis, all the markers of oxidative stress correlated significantly with maternal parameters (P < 0.005). On multivariate analysis, maternal albumin and hemoglobin accounted for maximum correlation with the markers of oxidative stress.
Intrauterine malnutrition is associated with significant oxidative stress in small for gestational age neonates born at term to malnourished mothers.
Oxidative stress occurs as a consequence of imbalance between the formation of oxygen free radicals and inactivation of these species by antioxidant defense system . Oxygen free radicals are incriminated in the causation of several neonatal diseases including bronchopulmonary dysplasia, retinopathy of prematurity, persistent ductus arteriosus, necrotizing enterocolitis, intracranial hemorrhage and hypoxic ischaemic encephalopathy [2–5]. The role of oxidative stress is also clear and well known in the pathogenesis of acquired malnutrition [6–8].
It is apparent that the intrauterine period of life is a very important period from a nutritional standpoint [9, 10]. The normal fetal growth is a result of complex interaction among the three components of maternal-placental-fetal unit. Nutritional status of the mother is the most important maternal factor leading to intrauterine growth retardation . Malnutrition involves deficiency of not only the macronutrients i.e. fats, proteins, carbohydrates but also results in subphysiological concentration of most micronutrients. Many antioxidant defense systems depend on micronutrients or are micronutrients themselves . Proteins provide amino acids for synthesis of antioxidant defense enzymes, reduced glutathione (GSH) and albumin (as sacrificial antioxidant protein). Therefore, one would expect a gross derangement of the antioxidant defense mechanisms in malnutrition. Small for gestational age (SGA) babies born at term to undernourished mothers provide a unique opportunity to have an insight into the mechanism and implications of the fetal growth retardation, secondary to intrauterine malnutrition. No study is available on the role of oxidative stress in term neonates born out of intrauterine growth retardation as a consequence of maternal malnutrition.
The body on account of susceptibility to oxidative insult, is naturally provided with an efficient antioxidant system. A series of enzymes also act as scavenging systems including superoxide dismutase (SOD), catalase and glutathione peroxidase. These enzymes are the first line of defense against reactive oxygen species and are generally referred to as primary antioxidants. Lipid peroxidation products are measured as an index of oxygen free radical generation. Malondialdehyde (MDA) is one such product generated in the tissues by free radical injury. Measurement of MDA provides a sensitive index of the lipid peroxidation and oxidative stress .
The present study was conducted to assess the status of oxidative stress in SGA babies born to undernourished mothers by estimating the blood levels of (i) free radical scavenger enzymes (superoxide dismutase and catalase), (ii) free radical scavenger (reduced glutathione) and (iii) lipid peroxidation product (malondialdehyde). We started with the hypothesis that SGA neonates babies born at term to malnourished women have deficient antioxidant defense mechanism and increased oxidative damage; this would be reflected by decreased level of free radical scavengers and excess of lipid peroxidation products.
This study was conducted over a period of 1 year in the Departments of Pediatrics and Biochemistry at University College of Medical Sciences and Guru Tegh Bagadur Hospital, New Delhi, India. The study protocol was approved by the Institutional Ethical Committee, and an informed signed consent was obtained from the expectant mothers.
The Study group consisted of 20 consecutive singleton small for gestational age (birth weight <10 percentile for gestational age), term neonates, otherwise healthy and born between 38–40 weeks to severely malnourished mothers. Mothers were selected if they (i) weighed < 50 kg (post-pregnancy) and/or had a height of < 145 cm, AND (ii) had a hemoglobin < 8 g/dL and/or serum albumin < 2.5 g/dL. These mothers were unbooked and reported to us in labor. The Control group included 20 age and sex matched healthy term neonates. These babies were appropriate for gestational age (weighing between 10th and 90th percentile) born to healthy mothers under regular antenatal care. These mothers had a hemoglobin > 10 g/dL, serum albumin > 3.5 g/dL, height > 150 cm, and a pre-pregnancy weight of more than 50 kg. Only those mothers having an uncomplicated antenatal and obstetric history, and known date of conception were included.
Women with an established medical risk factor for having reduced or excessive birth weight of the neonate such as, extremes of age (< 18 or > 44 years), hypertension, toxemia of pregnancy, renal disease, heart disease, diabetes, urinary tract infection, metabolic disorder, tuberculosis, smoking, and alcohol or chronic drug intake were excluded from both the groups. Infants were excluded if they had history of difficult delivery, fetal distress, one minute apgar score less than 8, congenital malformations, birth injury or any complication requiring special care e.g. sepsis, seizure, respiratory distress, congenital heart disease, hypothermia, hypoglycemia and hyperbilirubinemia etc. Infants born to mothers who received sedatives /anesthesia within 4 hours prior to delivery were also excluded. All included newborns were delivered by vaginal route, with vertex presentation.
Gestational age was assessed from the date of last menstrual period and concurrent clinical assessment using the New Ballard Score . For classifying infants as appropriate or small for gestation, reference of weight was made to Lubchenko's charts of intrauterine growth . Weight, length and circumferences of the mid-arm, head and chest were recorded for all neonates by the standard techniques within half an hour of birth. Ponderal index was calculated as weight (g)/[length (cm)]3 × 100.
Markers of oxidative stress
Four mL of heparinized venous blood sample was collected from the umbilical cord just after delivery of the neonate and processed for the isolation of erythrocytes and plasma. The red cells were stored at 4°C and all serum samples at -20°C until analysis. The red cell samples were processed within 12 hours. Serum malondialdehyde (MDA) was measured using the colorimetric method described by Satoh , based upon the reaction of thiobarbituric acid (TBA) with MDA, one of the aldehyde products of lipid peroxidation. The absorbance of the MDA-TBA adduct thus produced was measured at 532 nm wavelength using a spectrophotometer. The activity of superoxide dismutase (SOD) in erythrocytes was determined by the method described by Marklund and Marklund  as modified by Nandi and Chatterjee , which is based on the inhibition of pyrogallol autoxidation brought about by SOD. The catalase activity of the erythrocytes was determined by the method of Sinha , based on the principle that dichromate in acetic acid is reduced to chromic acetate when heated in the presence of H2O2 with the formation of perchromic acid as an unstable intermediate. Reduced glutathione (GSH) was estimated by a method based on the development of yellow color with 5, 5' dithiobis-2-nitrobenzoic acid (DTB), which was measured at 432 um using a spectrophotometer .
One-sample Kolmagorov-Smirnov test was done to confirm the assumption of normality in the two groups for four outcome variables i.e., MDA, catalase, superoxide dismutase and reduced glutathione. The levels of MDA, catalase, superoxide dismutase and reduced glutathione were compared between the Study and Control group by unpaired t test. The observed values for these four parameters of oxidative stress were correlated to the maternal anthropometry (weight, height, body mass index), hemoglobin and albumin; and the neonatal anthropometry (weight, length, head circumference, chest circumference, mid-arm circumference, ponderal index) by univariate analysis. For univariate analysis, Pearsonian correlation coefficient r was calculated. Multivariate analysis was carried out by multiple regression analysis using stepwise selection method. While levels of MDA, catalase, superoxide dismutase and reduced glutathione were taken as dependent variables, the independent variables that were considered simultaneously with each of the dependent variable included maternal weight, height, body mass index, hemoglobin and albumin. Significance of correlation was calculated by the analysis of variance and F test. P < 0.05 was considered significant. This level was also used as the cut-off for inclusion/exclusion in the stepwise selection method. The statistical analysis was carried out using SPSS version 10+ at the Division of Biostatistics and Medical Informatics at our Institution.
Maternal and neonatal characteristics in the two groups
Study Group (N = 20) Mean ± S.D
Control Group (N = 20) Mean ± S.D*
147.3 ± 3.6
156.1 ± 3.3
44.7 ± 5.5
56.8 ± 3.9
8.0 ± 0.9
11.6 ± 0.6
Serum albumin (g/dL)
2.5 ± 0.3
3.7 ± 0.2
2097 ± 145
2862 ± 290
48.0 ± 0.7
50.1 ± 1.4
Midarm circumference (cm)
9.5 ± 0.5
11.0 ± 0.3
Head circumference (cm)
33.7 ± 1.3
35.0 ± 0.5
Chest circumference (cm)
30.6 ± 1.3
31.9 ± 0.4
1.9 ± 0.1
2.2 ± 0.2
Biochemical markers of oxidative stress in enrolled newborns
Study Group (n = 20)
Control Group (n = 20)
Mean difference (95% CI)
2.78 (2.43, 3.13)
Superoxide dismutase (U/g Hb)
-293.23 (-336.80, -249.66)
Catalase (U/g Hb)
-0.82 (-0.97, -0.68)
Reduced glutathione (μ mol/g Hb)
-3.58 (-3.78, -3.38)
Simple correlation of oxidative stress markers with maternal and neonatal characteristics (n = 40)
Catalase (U/g Hb)
Superoxide dismutase (U/g Hb)
Reduced glutathione (μmol/g Hb)
Head circumference (cm)
Chest circumference (cm)
Midarm circumference (cm)
In the stepwise regression analysis for MDA, maternal albumin was found to be the most important predictor of lipid peroxidation in a multivariate set up (r = -0.885, SE = 0.171, F (1,38) = 136.6, P < 0.0001), followed by maternal hemoglobin (r = 0.925, SE = 0.586, F (2,37) = 109.577, P < 0.0001). No other maternal parameter was able to enter the equation after the second step. Maternal albumin was also found to be the most important predictor for catalase (r = 0.810, SE = 0.279, F (1,38) = 72.31, P < 0.0001) and reduced glutathione (r = 0.899, SE = 0.82, F (1,38) = 159.55, P < 0.0001) in their respective multivariate models. Hemoglobin was the only other variable that contributed to prediction of catalase and reduced glutathione. For superoxide dismutase, maternal weight entered the equation on Step 1 (r = 0.834, SE = 91.08, F (1,38) = 86.87, P < 0.0001). Body mass index was included as the next important parameter (r = 0.904, SE = 71.53, F (2,37) = 82.72, P < 0.0001). Two more variables entered the equation: i.e., albumin on Step 3 (r = 0.923, SE = 65.43, F (3,36) = 68.66, P < 0.0001), and height on Step 4 (r = 0.933, SE = 61.83, F (4,35) = 58.99, P < 0.0001). Hemoglobin did not add significantly to the prediction of superoxide dismutase in a multivariate set up (P = 0.229).
In this study, the oxygen free radical scavenging systems including superoxide dismutase, catalase, and reduced glutathione were lower (indicating deficient anti-oxidant defense mechanisms), and malondialdehyde (a measure of lipid peroxidation and oxidative damage) was higher in the cord blood of SGA neonates born to malnourished mothers as compared to AGA infants born to well nourished women, thus confirming our working hypothesis.
It is emphasized that these results apply specifically to the RBCs. The depressed superoxide dismutase, catalase and reduced glutathione levels in the undernourished group's RBCs can not be assumed to indicate a similar depression in enzymatic/non-enzymatic antioxidant protectants in other tissues, particularly the lungs. This is important because the lung, unlike RBCs has the ability to increase the production of key protective antioxidants when challenged by increased oxidative stress. Similarly, increased serum MDA values could well represent increased serum levels derived mainly or solely from unprotected RBCs alone.
The inclusion criterion in the present study were made stringent so as to exclude most factors other than the maternal malnutrition, which could have a bearing on the etiology of oxidative stress of the neonates. Only term neonates with an uneventful perinatal period were included. The cut off points for maternal height and weight clearly indicated that the included mothers had suffered chronic malnutrition, which was further supported by low hemoglobin and/or serum albumin levels. Infants delivered to these mothers had a ponderal index of < 2 indicating that the growth retardation was predominantly asymmetrical and had occurred due to intrauterine nutritional deprivation during the late second and third trimester. An exact dietary history of the mother taken during pregnancy could have validated our point further. However, this was not possible due to our study design and logistic constraints.
A study of the profile of antioxidant defense potential of erythrocytes during the late fetal development revealed that erythrocyte catalase, glutathione peroxidase and plasma vitamin E levels correlated positively with increasing fetal weight . In newborns, malnutrition may be a major element in the infant's intolerance against the harmful effects of free oxygen radicals . A low birth weight infant is also frequently exposed to the conditions associated with increased generation of free oxygen radicals such as reperfusion and hypoxia . Most of these data on LBW emanate from studies on preterms neonates [19, 21]. It would be of interest to show that the degree of oxidative stress far outweighs the antioxidant defense mechanisms in SGA babies born as a result of maternal malnutrition.
SGA newborns had double the concentration of MDA as compared to AGA controls indicating significant oxidative damage. The activity of superoxide dismutase and reduced glutathione in the Study group was 60–65% and 44% of that in the normal healthy controls. Similar result was obtained with respect to catalase. Previous studies have also measured values of these enzymes in cord blood of term and preterm infants and the normative data is in accordance with our values in the Control group [19, 22]. Maternal albumin and hemoglobin appeared to be the most important parameters to influence most of the markers of oxidative stress. This brings about an interesting hypothesis; biochemical indicators of nutrition are more closely linked to oxidative stress as compared to the anthropometric characteristics. On univariate analysis, neonatal mid-arm circumference was the most important determinant for MDA because MDA, a lipid peroxidation product is directly proportional to the amount of adipose tissue (generally measured by mid arm circumference).
However, the present study had certain limitations. While studying the role of antioxidants defenses in SGA babies born to undernourished mothers, total radical trapping capacity of anti-oxidants in plasma (TRAP)  should be studied and their levels compared to that in term AGA neonates. The concentrations of various other known antioxidants should also be measured and contribution of these antioxidants to TRAP should be calculated, which was not done in the present study. In order to understand the antioxidant defense system in the baby, a combined effect of the antioxidants should be measured, not just their individual cellular or plasma concentrations [24, 25]. Determination of oxidized/reduced glutathione ratio  could have further validated our results.
Our study design did not permit estimation of markers of oxidative stress in AGA babies born to undernourished mothers and IUGR babies born to well nourished mothers. With the present study, it cannot be concluded whether SGA, maternal malnutrition or even both are the cause or effect of neonatal oxidative stress. The changes in oxidative stress markers could still be due only to IUGR and not to low dietary intake of mothers. Further studies are needed to translate this association into a cause-effect relationship.
This study proves that there is a strong evidence of oxidative stress in the SGA babies born to undernourished mothers as evidenced by increased lipid peroxidation and reduced free oxygen radical scavenger system. The erythrocyte protective mechanisms against oxidative damage are more efficient in AGA babies of healthy mothers. From the present study, it is difficult to infer whether the oxidative stress is a cause or effect of intrauterine growth retardation. If oxidative stress can be proven to be the cause of intrauterine growth retardation, antioxidant therapy may be considered as a prophylactic/therapeutic modality for preventing intrauterine growth retardation secondary to maternal malnutrition. Micronutrient supplementation to pregnant women, who are at higher risk of having fetal growth retardation, may alleviate oxidative stress and promote anti-oxidant defense mechanisms in their offspring. This may act as a catalyst in increasing their size at birth and decreasing subsequent neonatal morbidity and mortality , especially in the first week of life.
- Rice-Evans C, Burdon R: Free radical lipid interactions and their pathological consequences. Prog Lipid Res. 1993, 32: 71-110. 10.1016/0163-7827(93)90006-I.View ArticlePubMedGoogle Scholar
- Drury JA, Nycyk JA, Baines M, Cooke RW: Does total antioxidant status relate to outcome in very preterm infants?. Clin Sci. 1998, 94: 197-201.View ArticlePubMedGoogle Scholar
- Lindeman JHN, Van Zoeren-Grobben D, Schrijver J, Speek AJ, Poorthuis BJHM, Berger HM: The total free radical activity of cord blood plasma in preterm and term babies. Pediatr Res. 1989, 26: 20-24.View ArticlePubMedGoogle Scholar
- Saugstad OD: Hypoxanthine as an indicator of hypoxia: its role in health and disease through free radical production. Pediatr Res. 1988, 23: 143-150.View ArticlePubMedGoogle Scholar
- Sullivan JL: Iron, plasma antioxidants and the "oxygen radical disease of prematurity". Am J Dis Child. 1988, 142: 1341-1344.PubMedGoogle Scholar
- Tatli MM, Vural H, Koc A, Kosecik M, Atas A: Altered antioxidant status and increased lipid peroxidation in marasmic children. Pediatr Int. 2000, 42: 289-292. 10.1046/j.1442-200x.2000.01217.x.View ArticlePubMedGoogle Scholar
- Ashour MN, Salem Sl, El-Gadbon HM, Elwan NM, Basu TK: Antioxidant status in children with protein-energy malnutrition (PEM) living in Cairo, Egypt. Eur J Clin Nutr. 1999, 53: 669-673. 10.1038/sj.ejcn.1600830.View ArticlePubMedGoogle Scholar
- Sive AA, Subotzky EF, Malon H: Red blood cell antioxidant enzyme concentrations in kwashiorkar and marasmus. Ann Trop Paediatr. 1993, 13: 33-38.PubMedGoogle Scholar
- Swain S, Singh S, Bhatia BD, Pandey S, Krishna M: Maternal hemoglobin and serum albumin and fetal growth. Indian Pediatr. 1994, 31: 777-823.PubMedGoogle Scholar
- Villar J, Belizan JM: The timing factor in the pathophysiology of the IUGR syndrome. Obstet Gynecol. 1982, 37: 499-506.Google Scholar
- Evans P, Halliwell B: Micronutrient: oxidants/antioxidant status. J Nutr. 2001, 85 (suppl 2): S67-S74.View ArticleGoogle Scholar
- Ballard JL, Khoury JC, Wedig K, Wang L, Filers-Walsman DL, Lipp R: New Ballard Score expanded to include extremely premature infants. J Pediatr. 1991, 119: 417-423.View ArticlePubMedGoogle Scholar
- Lubchenko LO, Hausman C, Dressler M, Boyd E: Intrauterine growth as estimated from live born birth weight data at 24 to 42 weeks of gestation. Pediatrics. 1963, 32: 793-800.Google Scholar
- Satoh K: Serum lipid peroxide in cerebrospinal disorder determined by a new colorimetric method. Clin Chim Acta. 1978, 40: 37-43.Google Scholar
- Marklund S, Marklund G: Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur J Biochem. 1974, 47: 469-474.View ArticlePubMedGoogle Scholar
- Nandi A, Chatterjee IB: Assay of superoxide dismutase activity in animal tissue. J Biosci. 1988, 13: 305-315.View ArticleGoogle Scholar
- Sinha AK: Colorimetric assay of catalase. Anal Biochem. 1972, 47: 389-394.View ArticlePubMedGoogle Scholar
- Beutler E, Duran O, Kelly BM: Improved method for the determination of blood glutathione. J Lab Clin Med. 1963, 51: 882-888.Google Scholar
- Huertas JR, Palomino N: Lipid peroxidation and antioxidants in erythrocyte membranes of full term and preterm newborns. Biofactors. 1998, 8: 133-137.View ArticlePubMedGoogle Scholar
- Frank L, Sosenko IRS: Failure of premature rabbits to increase antioxidant enzymes during hypertoxic exposure: increased susceptibility to pulmonary oxygen toxicity compared with term rabbits. Pediatr Res. 1991, 29: 292-296.View ArticlePubMedGoogle Scholar
- Saugstad OD: Oxygen toxicity in the neonatal period. Acta Paediatr Scand. 1990, 79: 881-892.View ArticlePubMedGoogle Scholar
- Yoshioka T, Kawada K, Shimada T, Mori M: Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygen toxicity in the blood. Am J Obstet Gynecol. 1979, 135: 372-376.View ArticlePubMedGoogle Scholar
- Lindeman JHN, Zoeren-Grobben DV, Schrijiver J, Speek AJ, Poorthuis BJHM, Berger HM: The total free radical trapping ability of cord blood plasma in preterm and term babies. Pediatr Res. 1989, 26: 20-24.View ArticlePubMedGoogle Scholar
- Willson RL: Free radical protection: why vitamin E, not vitamin C, beta carotene or glutathione?. In: Biology of Vitamin E. Ciba Foundation Symposium101. 1983, London: Pitmann Books, 19-44.Google Scholar
- Wayner DDM, Burton GW, Ingold KU, Barley DRC, Locke S: The relative contributions of vitamin E, urate, ascorbate and proteins to the total radical trapping antioxidant activity of human blood plasma. Biochem Biophys Acta. 1987, 924: 408-419. 10.1016/0304-4165(87)90155-3.View ArticlePubMedGoogle Scholar
- Nemeth I, Boda D: Blood glutathione redox ratio as a parameter of oxidative stress in premature infants with IRDS. Free Rad Biol Med. 1994, 16: 347-353. 10.1016/0891-5849(94)90036-1.View ArticlePubMedGoogle Scholar
- Hobbins J: Morphometry of fetal growth. Acta Paediatr. 1997, Suppl 423: 165-168.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2431/4/14/prepub
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