Fig. 3

KD sera upregulate the expression of Sema7A receptors in HCAECs. a The proinflamamtory cytokine mRNA expression and permeability of HCAECs cultured in 10% FBS-containing RPMI 1640 and subsequently stimulated with 10 µg/ml rhSema7A or not. b The proinflamamtory cytokine mRNA expression and permeability of HCAECs cultured in 20% KD sera-containing or 20% HC sera-containing RPMI 1640 and subsequently stimulated with 10 µg/ml rhSema7A or not. c The mRNA expression levels of plexin C1 and integrin β1 in HCAECs treated with 20% KD sera or HC sera. Data are expressed as M ± SEM from 3 separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s.:not significant. Sema7A: Semaphorin 7A; FBS: fetal bovine serum; PBS: phosphate buffered saline; KDS: sera from KD patients (RPMI 1640 containing 20% KD sera (pooled from 10 KD patients) ); HCS: sera from healthy controls (RPMI 1640 containing 20% healthy control sera (pooled from 10 healthy controls) ); KD: Kawasaki disease