Table 1 PCR program to amplify the rs1544410 fragment (BsmI) in the VDR gene
Number of cycles | steps | Temperatures (centigrade) | Time (second) |
|---|---|---|---|
1 | Pre-denaturation | °94 | 300 |
35 | Denaturation | °94 | 35 |
Annealing | °52 | 30 | |
Extension | °72 | 30 | |
1 | Final extension | °72 | 300 |