Table 1 Primers used for targeted NGS and Sanger sequencing
Amplified region | Length | Sequence of oligonucleotide(5’-3’) |
|---|---|---|
chr2:232957822–232,969,822 | 12,000 bp | F: CAGTCTTTCTTCTCCACTCCCACCCA R: CAGCCATCACAGCCACAGTTCAGG |
chr6:158724792–158,736,792 | 12,000 bp | F: TTCTGTCACTTCCTCCCTTTGCCTTCA R: CGCCTGCTACCCTACACCTTACCAACA |
chr2:232963168–232,963,968 | 800 bp | F: AATGCAGCTCTTTGATGGAACA R: TTCTACTGGCCTGTGACTTACTTG |
chr6:158730578–158,731,378 | 800 bp | F: CGGGCCAGGTAACCTCATG R: CAGGGTGGTGCTGTTGTGC |