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Table 2 Settings of the detectors for the detection of multiple pathogens

From: Burden of human metapneumovirus infections among children with acute respiratory tract infections attending a Tertiary Care Hospital, Kathmandu

PP mix

Pathogens

Dye

Detection wavelength (nm)

FluRhino PP (Probe/Primer mix for FLUA, RV, FLUB, H1N1)

FLUA (Influenza A)

green

520

RV (Human rhinoviruses)

yellow

550

FLUB (Influenza B)

orange

610

H1N1 (Influenza A subtypes))

red

670

Cor PP (Probe/Primer mix for Cor299, Cor 63, HKU, Cor 43)

Cor 229 (Human coronavirus-299)

green

520

Cor 63 (Human coronavirus-63)

yellow

550

HKU ((Human corona virus-HKU1)

orange

610

Cor 43 (Human coronavirus-43)

red

670

ParaEAV PP (Probe/Primer mix for HPIV2-4, IC)

HPIV3 (Human parainfluenza virus 3)

green

520

HPIV2 (Human parainfluenza virus 2)

yellow

550

HPIV4 (Human parainfluenza virus 4)

orange

610

IC (EAV)- Internal control-Equine arteritis virus)

red

670

BoMpPf1 PP (Probe/Primer mix for HPIV1, HMPV A/B, HboV, Mpneu)

HPIV1 (Human parainfluenza virus 1)

green

520

HMPV A/B (Human Metapneumovirus A & B)

yellow

550

HBoV (Human Boca virus)

orange

610

Mpneu (Mycoplasma pneumoniae)

red

670

RsEPA PP (Probe/Primer mix for HRV A/B, HPeV, EV, HAdV)

HRSVA/B (Human respiratory syncytial virus A & B)

green

520

HPeV (Human Parechoviruses)

yellow

550

EV (Entero virus)

orange

610

HAdV (Human adenovirus)

red

670