Table 1 PCR primers used for the IKBKG deletion-specific PCR
Nucleotide | ||
|---|---|---|
First PCR | Forward primer | AGGACAGCCTTGCTGGATCTTAC |
Reverse primer | GTCTTCCTTCCTGAAGCTGTGTCT | |
Nested PCR | Forward primer | GAGGCAGATGCAGATGAGGAGG |
Reverse primer | CTACCGAGGCAAGAAGATCGCT | |
Quantitative PCR | Probe | TTCATGCCATTCTCCTGCCTCAGCCTCC |
Forward primer | TGAGGGAGAGATTCACGAAGAAGT | |
Reverse primer | CTGCGGCCATCTGTTTTTGC |