Table 1 The investigation content required at baseline, follow-up of the prospective cohort study
Items | Baseline | First visit | Second visit |
|---|---|---|---|
Children’s health and family environment questionnaire (all participants) | |||
 Lifestyle | √ | √ | √ |
 Family environment | √ | √ | √ |
 Parental information | √ | √ | √ |
 Early life information | √ | √ | √ |
Physical examination (all participants) | |||
 Anthropometric measures | √ | √ | √ |
 Sexual development | √ | √ | √ |
Biological samples (participants selected for case-control study) | |||
 Blood | √ |  |  |
 Urine | √ | √ | √ |
 Feces | √ |  |  |
Laboratory examination (participants selected for case-control study) | |||
 Endogenous hormones | √ | √ | √ |
 Genes | √ |  |  |
 EEDs | √ |  |  |
 Gut microbiota | √ |  |  |