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Table 4 Microorganisms detected in 525 children and adolescents with community acquired pneumonia and 61 controls, by mPCR, UA, IFA and acute serology IgM for M. pneumoniae

From: Etiology and the challenge of diagnostic testing of community-acquired pneumonia in children and adolescents

Microorganism, diagnostic test

CAP group (N = 525)

n (%)

Control group (N = 61)

n (%)

Streptococcus pneumoniae:

 mPCR

435 (82.9)

30 (49.2)

 UAa

92 (17.9)

4 (8)

Haemophilus influenzae. mPCR

359 (68.4)

32 (52.5)

Respiratory syncytial virus A and B:

 IFA

39 (7.4)

4 (6.5)

 mPCR

111 (21.1)

5 (8.2)

Parainfluenza virus 1, 2, 3, 4:

 IFA

33 (6.3)

6 (9.8)

 mPCR

68 (12.9)

6 (9.8)

Rhinovirus A/B/C. mPCR

111 (21.1)

6 (9.8)

Influenza A and B:

 IFA

12 (2.3)

2 (3.3)

 mPCR

47 (8.9)

5 (8.2)

Mycoplasma pneumoniae:

 mPCR

54 (10.3)

2 (3.3)

 Acute serology IgM

170 (32.4)

23 (37.7)

Metapneumovirus. mPCR

37 (7.0)

0

Adenovirus:

 IFA

19 (3.6)

6 (9.8)

 mPCR

4 (0.8)

0

Coronavirus 229E/NL63 and OC43. mPCR

15 (2.9)

0

Bordetella pertussis. mPCR

8 (1.5)

3 (4.9)

Enterovirus. mPCR

6 (1.6)b

0

Chlamydophila pneumoniae. mPCR

1 (0.2)

0

Bocavirus 1/2/3/4. mPCR

2 (0.5)b

0

Legionella pneumophila:

0

1 (1.6)

  mPCR

0

1 (1.6)

  UA

0

0

  1. IFA indirect immunofluorescence assay, UA Urinary antigen, mPCR multiplex PCR
  2. a UA: n = 515 in CAP group, and n = 50 in control group
  3. b mPCR for Enterovirus and Bocavirus: n = 379 in the CAP group and n = 61 in the control group (commercial diagnostic kit changed during the study to include these two new viruses, from RV12 to RV15 Seeplex® ACE Detection)