Fig. 2

Identification of bacterial pathogens using the melting temperature mapping method. a Amplification of the 16 S rDNA gene extracted from pus, positive control (Escherichia coli) and negative control (highly pure water) indicated by the red line, blue line and green line, respectively, in the 1st PCR. b Melting curves of the seven amplicons in the 2nd PCR. c The seven melting temperatures of the amplicons were mapped, and their plot matches the plot of Streptococcus pneumoniae in the database