Fig. 1

Representative agarose gel (2%) showing PCR product for 1st and 2nd rounds to detect 198 bp and 97 bp of B1 gene specific to Toxoplasmosis, respectively. In which, M represents low molecular weight DNA marker (50-1500 bp), lanes 1 & 2 represent first round and lanes 3 & 4 represent second round of nested PCR, however it shows other lower non-specific bands