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Table 3 Twelve isolates were analyzed by WGS. The 12 isolates consisted of seven non-typeable isolates and five MDR defined isolates

From: Pneumococcal carriage among children under five in Accra, Ghana, five years after the introduction of pneumococcal conjugate vaccine

Isolate number

MLST type (ST)a

Clonal complex (CC)/ nearest ST

Genotype/Capsular locus top hit (Nearest match)

Serotype

Pen/Tetra/SXT/Ery (Phenotypical)

tet(M)

ermB

PBP 1a

PBP 2b

PBP 2x

Predicted value*/Phenotype value

G10

(2–5–36-12-2-21-?)

ST908/ST11041

Top hit: Genotype 38

NT

I/S/R/S

–

–

38

25

43

MIC =? / MIC = 1

C131

(2–5–36-12-2-21-?)

ST908/ST11041

Top hit: Genotype 38

NT

I/S/R/S

+ (Tn916)

–

38

25

43

MIC =? / MIC = 2

C28

(2–5–36-12-2-21-?)

ST908/ST11041

Top hit: Genotype 38

NT

I/S/R/S

+ (Tn916)

–

38

25

43

MIC =? / MIC = 2

G11

344

Singleton

Top hit: Genotype 38

NT

I/R/R/S

+ (0)c

–

55

16

150

MIC =? / MIC = 0.12

C139

(2–149–1-12-6-494-14)

ST11264

Top hit: Genotype 14

NT

S/R/I/S

+ (Tn916)

–

24

73

192

MIC =? / Oxa sentitive

C140

9735

CC63

Top hit: Genotype 14

NT

S/R/R/S

+ (Tn916)

–

24

73

192

MIC =?/Oxa sentitive

G7

(2–37–36-29-17-21-14)

ST9735

Top hit: Genotype 14

NT

I/R/R/S

+ (Tn916)

–

24

73

192

MIC =? / MIC = 0.12

D012

2613

Singleton

Genotype 15A

15A

I/R/S/R

+ (Tn916)

+ (0)c

34

89

147

MIC = 2/ MIC = 2

G14

320

CC320

Genotype 19A

19A

I/R/R/R

+ (0)c

+ (0)c

13

11

16

MIC = 4/ MIC = 1

G27

320

CC320

Genotype 19A

19A

I/R/R/R

+ (0)c

–

13

11

16

MIC = 4/ MIC = 1

G28

320

CC320

Genotype 19A

19A

I/I/I/R

+ (0)c

+ (0)c

13

11

16

MIC = 4/ MIC = 1

C88

320

CC320

Genotype 19A

19A

I/R/R/R

+ (0)c

+ (0)c

13

11

16

MIC = 4/ MIC = 1

  1. aThe MLST locus allele is presented as (aroE-gdh-gki-recP-spi-xpt-ddl)
  2. * Predicted level of resistance to penicillin according to CDC (https://www.cdc.gov/streplab/pneumococcus/mic.html)
  3. ? Predicted value is not presented (https://www.cdc.gov/streplab/pneumococcus/mic.html)