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Fig. 5 | BMC Pediatrics

Fig. 5

From: Necrotizing enterocolitis leads to disruption of tight junctions and increase in gut permeability in a mouse model

Fig. 5

Biotin permeability assays in control and NEC intestines. a: Sulfo-NHS-LC-Biotin was injected into the intestinal lumen of 7-day old control (CON) or NEC pups. The tissue sections were stained with Texas red-conjugated streptavidin. Biotin was mainly kept at the epithelial surface of control intestines (arrowhead) due to the intact epithelial barriers. No barrier leakage was observed in control intestines. In contrast, the leakage was clearly detected in NEC intestines. Biotin stained the intercellular space (arrow) and connective tissue (Asterisk). V: Villus. b: The higher magnification of the images. L: Lumen. Scale bar: 50 μm in A and 20 μm in B

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BMC Pediatrics

ISSN: 1471-2431

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