Fig. 3

Protein expression levels of control and NEC intestines. a: Representative western blot membrane showing claudin-2, -3, -4 and -7 expression levels in control (CON) and NEC intestines. Intestinal tissues from 7-day control and NEC pups were collected at the end of experiments. Tissue lysates were solubilized in RIPA buffer and subjected to western blotting. b: PARP, NF-κB and TGF-β expression levels in control (CON) and NEC intestines. A total of 30 μg protein for each sample were loaded on the SDS-polyacrylamide gel. Membranes were blotted against specific antibodies. Actin served as a loading control. Three independent experiments were performed