Fig. 1
Representative standard curve, amplification plot, and dissociation curve of patients’ samples. a The assay was linear for DNA copy numbers (R2 = 0.9892). b The amplification plot showed no regular amplification for the standard diluents. c The dissociation curve showed a single melting temperature of specific products generated with a standard template. These dissociation curves indicated that the reactions were free of primer-dimer or any other spurious products. d Similar amplification plots were observed after quantitative polymerase chain reaction using patients’ samples. The amplification curves generated from human samples were paralleled with the curves from standards and were in the range of amplification plots for standard diluents