Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms

Table 1 Design of the one-step triplex qRT-PCR assays for the detection of RSV, hMPV and Internal Control

Virus	Target gene	Name of primer or probe	Sequence (5′ → 3′)	Position
RSV	Nucleoprotein	RSV-F	TGATACACTSAACAAAGATCAACTTCTG	27–54^a
		RSV-R	TCTCCTGTGCTMCGTTGRAT	73–92^a
		RSV-probe	VIC-CATCCAGCAAATACAC-MGBNFQ	56–71^a
hMPV	Fusion protein	hMPV-F	TCAGAATGCAGGGTCAACTGTT	156–177^b
		hMPV-R	GACATGGTCTCCTCTTGTTTCACA	199–222^b
		hMPV-probe	FAM-CAAGCTTCCCGTTCTCAGCC-MGBNFQ	179–197^b
Internal Control	GAPDH	GAPDH-F	GAAGGTGAAGGTCGGAGTC	108–126^c
		GAPDH-R	GAAGATGGTGATGGGATTTC	314–333^c
		GAPDH-probe	NED-CAAGCTTCCCGTTCTCAGCC-MGBNFQ	285–304^c

ISSN: 1471-2431