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Fig. 1 | BMC Pediatrics

Fig. 1

From: Using a practical molecular capsular serotype prediction strategy to investigate Streptococcus pneumoniae serotype distribution and antimicrobial resistance in Chinese local hospitalized children

Fig. 1

The Streptococcus pneumoniae serotype prediction algorithm – a strategy based on cpsB sequetyping and selected mPCR. Step 1. The cpsB sequetyping was performed on all 193 S. pneumoniae isolates. Sequencing results compared with cpsB sequetyping database. 21 different sequetypes were identified; included serotype-specific sequetypes, sequetypes shared by multiple serotypes and novel cpsB sequetypes. Step 2. Modified and selected USA CDC sequential multiplex PCR to double check, or resolve discrepant results, or identify those that shared the same cpsB sequetype. Step 3. Serotypes 6A-6D specific PCRs were performed for serogroup 6 isolates. Step 4. Submit all novel cpsB sequetypes to GenBank and update cpsB sequetyping database

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BMC Pediatrics

ISSN: 1471-2431

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