Figure 4

The panels on the left show representative photomicrographs of IGF-I protein expression (4A), 50× (denoted by the brown color and arrows); ^a p < 0.04 Rapamycin (2 weeks) vs Control (2 weeks); ^b p < 0.03 Rapamycin (2 weeks) vs Rapamycin (4 weeks); ^c p < 0.03 Control (2 weeks) vs Control (4 weeks). IGFBP3 protein expression (4B, denoted by arrows and brown color), 50×; ^ap < 0.002 Rapamycin (2 weeks) vs Control (2 weeks); ^bp < 0.03 Rapamycin (2 weeks) vs Rapamycin (4 weeks). Type × collagen (col10a) protein expression localized only to the hypertrophic chondrocytes (4C, denoted by purple color and brackets), 20×; ^ap < 0.005 Rapamycin (2 weeks) vs Control (2 weeks); ^bp < 0.02 Rapamycin (4 weeks) vs Control (4 weeks). The panels on the right show the quantification of the protein expression for IGF-I (upper panel); IGFBP3 (middle panel); and type × collagen (lower panel) after 2 weeks and 4 weeks in Rapamycin and Control groups expressed as number of labeled cells to the total number of cells in the appropriate zone (Labeling Index).