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Table 2 Sequence Protocol for TIPIT

From: TIPIT: A randomised controlled trial of thyroxine in preterm infants under 28 weeks gestation: Magnetic Resonance Imaging and Magnetic Resonance Angiography protocol

Sequence Scan Parameters Target area
1. Midline sagittal T1 weighted Turbo Spin Echo (TSE) TR = 337 ms, TE = 18 ms Slice thickness = 3 mm, 0.3 mm gap Scan Duration = 2 minutes Anatomical alignment and measurement of pituitary height
2. Coronal heavily T2 weighted TSE (whole brain) TR = 5750 ms, TE = 150 ms Slice thickness = 1.5 mm, contiguous Scan Duration = 5 minutes Measurement of whole brain volume (cerebrum/cerebellum) and measurement of specific brain structures using stereology:
      • caudate
      • hippocampus
      • thalamus
      • prefrontal cortex
      • inferior frontal gyrus
      • temporal lobe
3. MR Angiography TR = 25 ms, TE = 6.9 ms Slice thickness = 0.6 mm, 3D Time of Flight (TOF) Multiple Overlapping Thin Slab Acquisition (MOTSA) with a SENSE factor of 2.00. Isotropic resolution = 0.6 × 06 × 06 mm3 Scan Duration = 6 minutes Blood vessel diameter, tortuosity, and density
4. Diffusion tensor imaging 2D single shot spin-echo Echo-Planar Imaging (SE-EPI) with SENSE factor of 2.5. 24 contiguous axial slices of 3 mm thickness with in-plane isotropic resolution of 2 mm. TR/TE = 3350/74 ms. 32 gradient directions with a b-factor of 700 s/mm2. 2 averages. Scan Duration = 3 minutes 47 s. Measurement of degree of mean diffusivity and fractional anisotrophy (as markers of water content and myelination) in
      • posterior limb of internal capsule
      • centrum semiovale
      • anterior and posterior corpus callosum
      • frontal white matter
      • occipital white matter
5. Axial T2-weighted TSE TR = 3023 ms, TE = 150 ms. Axial slices of 3 mm thickness with an in-plane isotropic resolution of 0.7 mm Scan Duration = 3 minutes In same slice location as DTI to aid region drawing