Similar DNA methylation levels in specific imprinting control regions in children conceived with and without assisted reproductive technology: a cross-sectional study

Table 3 Adjusted regression model results by infertility diagnosis

Gene region of interest	Female vs. Both^a		Male vs. Both^a		Unexp vs. Both^a		Group comparison p-value
	Difference^b	(95% CI)	Difference^b	(95% CI)	Difference^b	(95% CI)
Lymphocyte samples
KvDMR	0.03	(-1.75,1.81)	-0.21	(-2.84, 2.43)	-2.71	(-6.40, 0.98)	0.51
H19 CTCF3	-0.96	(-2.96, 1.05)	-1.10	(-3.26, 1.06)	-2.62	(-5.99, 0.76)	0.56
H19 CTCF6	-0.89	(-3.82, 2.04)	1.01	(-2.68, 4.71)	-1.98	(-8.61, 4.66)	0.72
IGF2DMR0	-1.26	(-3.24, 0.71)	-1.03	(-3.59, 1.53)	-3.45	(-6.87,-0.04)	0.40
IGF2R	0.43	(-4.02, 4.88)	-3.40	(-7.48, 0.67)	-5.92	(-13.06, 1.22)	0.39
Buccal samples
KvDMR	-5.50	(-10.86,-0.15)	-4.03	(-9.42, 1.37)	-7.40	(-14.66,-0.15)	0.14
H19 CTCF3	-1.63	(-5.29, 2.02)	-1.84	(-5.95, 2.27)	-4.72	(-9.41,-0.03)	0.37
H19 CTCF6	-3.52	(-8.37, 1.33)	-0.77	(-7.57, 6.02)	-5.16	(-12.33, 2.00)	0.30
IGF2DMR0	0.17	(-2.86, 3.19)	0.57	(-2.64, 3.78)	-1.34	(-5.93, 3.26)	0.82
IGF2R	0.76	(-1.80, 3.32)	1.31	(-0.94, 3.55)	-3.64	(-10.24, 2.95)	0.54

a Infertility diagnoses were divided into the following groups: Female factors (Female) n = 21, Male factors (Male) n = 17, Both male and female factors (Both) n = 16, and Unexplained (Unexp) n = 6
b Adjusted for pyrosequencing run, child's age, child's birth weight, child's sex, maternal age, maternal education, and household income

ISSN: 1471-2431