Table 1 Summary of HIV-1 pol FP-DBS PCR assay performed on DBS spiked with low copy number ACH2 cells
Cell count Copies/ml | pol real-time copies/rx (on ACH2 used to spike) avg 3 tests | No. positive (%) qualitative PCR on DBS lysate | Avg % D1 or D2 | Avg % both days (D1 and D2) |
|---|---|---|---|---|
10(A)D1 | 11.4 | 40(100) | 98.7 | 98.1 |
10(B)D1 | 6.5 | 39(97.5) | ||
10(A)D2 | 17.5 | 38(95) | 97.5 | |
10(B)D2 | 12.5 | 40(100) | ||
5(A)D1 | 12.6 | 26(65) | 72.5 | |
5(B)D1 | 4.95 | 32(80) | 72.5 | |
5(A)D2 | 3.5 | 25(62.5) | 72.5 | |
5(B)D2 | 3.6 | 33(82.5) | ||
2(A)D1 | 1.1 | 27(67.5) | 75 | 46.9 |
2(B)D1 | 3.05 | 33(82.5) | ||
2(A)D2 | 1.5 | 11(27.5) | 18.8 | |
2(B)D2 | 1.3 | 4(10) | ||
1(A)D1 | 1.6 | 18 (45) | 56.2 | 38.7 |
1(B)D1 | 0.8 | 27(67.5) | ||
1(A)D2 | 0.1 | 9(22.5) | 21.2 | |
1(B)D2 | 3.3 | 8(20) |