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Figure 2 | BMC Pediatrics

Figure 2

From: Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting

Figure 2

Experimental approach for performance of HIV-1 gag and pol FP-DBS PCR assay for detection of HIV-1 infection from FP-DBS obtained from HIV-1 positive infants as defined by Roche Amplicor v1.5 assay. 186 recently collected FP-DBS that were determined in the prior ~month to be HIV-1 positive by Roche Amplicor v1.5 assay were tested by HIV-1 gag and pol PCR assays. Of these 178 FP-DBS from the infants were identified as HIV-1 positive. Retesting of subsequent blood samples from the 8 discordant infants showed that they were HIV-1 negative. Thus, the sensitivity of HIV-1 pol PCR assay was found to be 100% (178 of 178) for detection of confirmed HIV-1 positive infants from FP-DBS.

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BMC Pediatrics

ISSN: 1471-2431

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