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Figure 1 | BMC Pediatrics

Figure 1

From: Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting

Figure 1

Gel picture of amplified products using 'in-house' HIV-1 gag and pol FP-DBS PCR assay. Gel picture of the HIV-1 pol (top panel - 160bp) and gag (lower panel - 152bp) PCR products. Lanes labeled from 1- 10 are PCR products from the ACH copy number controls: 1-2 are 100 HIV copies; 3-4 are 10 copies; 4-8 are 2 copies; 9 and 10 are negative controls using DNA from an uninfected T cell cell-line and water as the PCR template, respectively. PCR results from two samples of DBS-FP from infants who tested positive with the Roche Amplicor assay are labeled as A1-A4 and B1-B4, with 1-4 indicating quadruplicate tests. Based on the results show, both infants would be defined a HIV-1 DNA PCR positive according to testing algorithm for the' in-house' gag and pol FP-DBS FP PCR. The first and last lane in the gel picture (labeled M) in both the panels is the molecular weight marker - Hyperladder IV.

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BMC Pediatrics

ISSN: 1471-2431

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