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Table 1 Primers used in multiplex PCR

From: One third of middle ear effusions from children undergoing tympanostomy tube placement had multiple bacterial pathogens

 Primer Name

Nucleotide Sequence

Predicted Amplicon Sizea

H. influenzae FWD

5′-CGTATTATCGGAAGATGAAAGTGC-3′

525 base pairs

S. pneumoniae FWD

5′-AAGGTGCACTTGCATCACTACC-3′

484 base pairs

A. otitidis FWD

5′-GGGGAAGAACACGGATAGGA-3′

264 base pairs

M. catarrhalis FWD

5′-CCCATAAGCCCTGACGTTAC-3′

237 base pairs

Universal REV

5′-CTACGCATTTCACCGCTACAC-3′

 
  1. aWhen the designated FWD primer is used in conjunction with the Universal REV primer.
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BMC Pediatrics

ISSN: 1471-2431

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