Fig. 3
Graphical illustration of optimal sample size calculation for the study protocol. The plot illustrates the range of sample size values and corresponding test power. The power anova test setting was used to compute the sample size (α (significance) = 0.05; statistical power = 0.8; knumberofgroups = 6; f (effect size) = 0.25). Dashed blue line indicates the optimal sample size (n) that needs to be collected for the detection of significant results according to pre-determined thresholds in each experimental group