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Table 3 Microbiological and Biochemical test

From: Developing shelf-stable Microbiota Directed Complementary Food (MDCF) prototypes for malnourished children: study protocol for a randomized, single-blinded, clinical study

Parameters for Microbiological tests

Test name

Method

Acceptable limit

CFU/gm

Total plate count/Aerobic bacteria/APC

USFDA/BAM

<10,000

Total coliform/E. coli

USFDA/BAM

<10

Yeast and Mold Count

USFDA/BAM

<10

Salmonella

USFDA/BAM

Nil

Shigella

USFDA/BAM/ ISO

Nil

Bacillus cereus

USFDA/BAM/ ISO

<10

Staphylococci

USFDA/BAM/ ISO

<10

Enterobacteriaceae

USFDA/BAM/ ISO

<10

Parameters for Biochemical tests

 Test name

Acceptable limit

 pH

6.5-7.5

 Moisture

<2.5% for dry food product