Fig. 6From: Clinical and genetic analysis of a case with centronuclear myopathy caused by SPEG gene mutation: a case report and literature reviewThe result of cDNA PCR gel electrophoresis showed that the novel c.3715 + 4C > T splicing mutation produced an abnormal transcript; The PCR product of normal transcript was 608 bp, and that of abnormal transcript was 384 bp. SPEG cDNA forward primer: 5′-GAGCTGCCCGTTTCGACTGCAA-3′. SPEG cDNA reverse primer: 5′-GACCACATCTGTGACATACAGG-3′Back to article page