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Fig. 1 | BMC Pediatrics

Fig. 1

From: Cholinergic innervation and ganglion cell distribution in Hirschsprung’s disease

Fig. 1

Sampling and study design. a Intraoperative aspect of rectosigmoid colon during TAPT procedure. There was a notable change of diameter at the beginning of TZ (TZ 0 cm). White lines mark the biopsy sites at 5, 10 and 15 cm proximal to the beginning of the transition zone (TZ 0 cm). (Asterisk indicating aboral/distal end of colon, arrowhead the oral/proximal resection region). b Swiss roll specimens stained for H&E, AChE, NADH and LDH (asterisk and arrowhead as described in (a)). c First, we analyzed the swiss rolls of the full-length specimens and located areas with each of the four grades of the AChE-Score. In the same region, we counted GCs. d Second, we analyzed the specimen TZ 0, 5, 10 and 15 cm for their respective AChE-Score (AChE) and counted the GC/mm in the corresponding area (NADH)

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