Fig. 1

a. and b. Hepatocellular edema, multinucleated hepatocytes (arrows), accumulation of bile pigment inside the bile canaliculi and hepatocytes (black arrowheads) and mild portal infiltration of neutrophil and eosinophil granulocytes (white and grey arrowheads respectively) is presented. Original magnification: 60 x. pv: portal vein, cv: central vein. c. Immunohistochemical stainings for BSEP (BS transporter) and MRP2 (canalicular bilirubin and glutathione transporter) are shown in the control and patient’s liver specimens. As shown, BSEP-positive inclusions retained in the hepatocellular cytoplasm and no transporter was detected on the canalicular membrane. The internal control MPR2 shows preserved canalicular expression. Original magnification 100x. The specificity of immunohistochemical staining was confirmed by negative control (not shown)