Table 1 Biological and Questionnaire Data Collected at Each Time Point
Measure | Time Point | Group | Type of Assay/Analysis |
|---|---|---|---|
Stool | 1-week, 3-, 6-, 12-, & 18 month | Infant | Gut microbiome 16 rRNA gene sequencing |
Blood | 1-week & 18 month | Infant | Plasma cytokines |
Saliva | 3-, 6-, 12-, & 18 month | Infant, Mother | Diurnal cortisol, collected 3 times (at wake, 30 min after awakening, and 8 pm) & Salivary oxytocin |
Buccal | 1-week, 3, 6-, 12- month | Infant | HLA genotypes & DNA methylation |
Questionnaires | 1-week, 3-, 6-, 12-, & 18 month | Mother | Perceived stress, depression symptoms, anxiety |
Infant Feeding Survey | 1-week, 3-, 6-, 12-, & 18 month | Mother | Maternal report of infant feeding sources |
Infant Food Diary | 3,6 and 12 month | Mother | Record of infant food consumption in 24 h period |
Infant + Maternal Sleep & health | 3-, 6-, 12-, & 18 month | Mother | Maternal report of infant and mother’s sleep patterns and illnesses, diagnoses, medications |
Parental Bonding | 6 months | Mother | Maternal report of their mother’s parenting style for first 16 years of life. |
Stressful life events & Neighborhood safety | 12-month | Mother | Measures on new life stressors and neighborhood and home safety |
Sociodemographic | 3, 12-month | Mother | Questionnaire on sociodemographic variables |
Videotaped Observation | 3-, 6-, 12-, & 18 month | Infant, Mother | Maternal-infant interaction style |
NNNS | 1-week | Infant | Infant neurodevelopment |
Infant Cry | 1-week | Infant | Cry quality for neural integrity |
Bayley Scales | 3-, 6-, 12-, & 18 month | Infant | Infant cognitive and social-emotional development |
Child Growth Measures | 1-week, 3-, 6-, 12-, & 18 month | Infant | Infant growth trajectory |
Maternal Cognitive Assessment | Once at any visit | Mother | Measures memory, visual processing and information retrieval |