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Fig. 5 | BMC Pediatrics

Fig. 5

From: Functional characteristics of circulating granulocytes in severe congenital neutropenia caused by ELANE mutations

Fig. 5

Characterization of bone marrow and blood cells from one SCN4 patient with heterozygous mutations in the G6PC3 gene. a One representative image (out of 6) of bone marrow fluid stained with Wright’s stain from the SCN4 patient. b The bar graph shows the percentage (mean + SD; analysed at two separate occasions) of neutrophils (black bars) and eosinophils (grey bars) in different development stages in bone marrow samples; promyelocytes (PM), myelocytes (MC), metamyelocytes (MM), band cells (BC), and segmented cells (SC), from the SCN4 patient. c The histograms show the auto fluorescence of unstained Ficoll-Paque enriched granulocytes from two healthy controls (HC, grey), and the SCN4 patient (black). d-f NADPH oxidase derived ROS production by Ficoll-Paque enriched granulocytes from the SCN4 patient (black line) and two HC (grey lines) was measured in a plate reader using (d-e) luminol-amplified CL, and (f) isoluminol-amplified CL. The traces show (d-e) intracellular ROS production induced by (d) ionomycin (0.5 μM), (e) PMA (50 nM), and (f) extracellular ROS production induced by PMA (50 nM)

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