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Fig. 3 | BMC Pediatrics

Fig. 3

From: Functional characteristics of circulating granulocytes in severe congenital neutropenia caused by ELANE mutations

Fig. 3

Diminished intracellular NADPH-oxidase derived ROS production in Ficoll-Paque enriched SCN1 granulocytes. ROS-production by granulocytes was measured in a plate reader using (a-b) isoluminol-amplified CL to measure extracellular ROS, and (c-f) luminol-amplified CL to measure intracellular ROS. The left lane shows one representative trace of ROS production (RLU) over time (seconds; s), and the right lane shows the peak ROS-production by SCN1 granulocytes (black line; black dots) and healthy control granulocytes (HC; grey lines; grey dots). Samples were stimulated with (a-b) PMA (50 nM, n = 6; P01, P03-P05, P07 and P09), (c-d) PMA (50 nM, n = 6; P01-P05 and P07), or (e-f) ionomycin (0.5 μM, n = 7; P01-P07). Statistical analysis in b, d, and f was performed by an unpaired Student’s t-test

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