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Fig. 2 | BMC Pediatrics

Fig. 2

From: Functional characteristics of circulating granulocytes in severe congenital neutropenia caused by ELANE mutations

Fig. 2

Eosinophils dominate Ficoll-Paque enriched SCN1 granulocyte fractions. Flow cytometry analysis was performed on (a-b) Ficoll-Paque enriched granulocytes (Gran) and (c) PBMCs to examine the number of neutrophils (Neu) and eosinophils (Eos). a Upper panel; Granulocytes from SCN1 patients (P05 and P06) and healthy controls (HC; n = 2) were gated based on side scatter (SSC) and forward scatter (FSC). Lower panel; The percentage of the different granulocytes (Eos; CD49d+CD16b− and Neu; CD49d−CD16b+) are shown. b Granulocytes from one SCN1 patient (P01, upper panel) and one HC (lower panel) were gated for the percentage of myeloid cells (CD11b+CD45+), eosinophils (CD15+CD16−) and neutrophils (CD15+CD16+). The eosinophil population was confirmed by expression of CCR3 and Siglec-8 (shown in red) as compared to the CCR3−Siglec-8− neutrophil population (shown in black). c The PBMCs from the same SCN1 patient (P01, upper panel) and HC (lower panel) were evaluated for the content of neutrophils by examining the percentage of myeloid cells (CD11b+CD45+), granulocytes (CD14−CD15+), eosinophils (CCR3+CD16−; red) and neutrophils (CCR3−CD16+; black)

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