Fig. 4
Detection of VDR gene BsmI polymorphism. PCR amplification of genomic DNA was carried out and the products of amplification were cleaved with restriction enzyme MvaI (details given in Methods). A: lane 1, phiX174 HaeIII cut Mr markers; lane 2, uncleaved PCR product; lanes 3–7, cleavage products from subjects with BB genotype. B: lane 1, ϕX174 HaeIII cut Mr markers; lane 2, uncleaved PCR product; lane 3,5, cleavage products from subjects with bb genotype; lanes 4, cleavage products from subjects having Bb genotype. The numbers on the side are sizes (bp) of the characteristic bands resulting from PCR-RFLP analysis of the subjects having different genotypes. The restriction enzyme cleavage products were analyzed on 2% agarose gel and visualized under UV light after staining with Ethidium bromide