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Fig. 3 | BMC Pediatrics

Fig. 3

From: Relationship of four vitamin D receptor gene polymorphisms with type 1 diabetes mellitus susceptibility in Kuwaiti children

Fig. 3

Detection of VDR gene ApaI polymorphism. PCR amplification of the genomic DNA was carried out and products of amplification were cleaved with restriction enzyme ApaI (details given in Methods). Lane 1, phiX174 HaeIII cut Mr markers; lane 2, uncleaved PCR product; lanes 3,4,8,9, products from subjects with heterozygous GT genotype; lanes 5–6, products from subjects with homozygous TT genotype; lane 7, products from homozygous GG genotype. The numbers on the side are sizes (bp) of the characteristic bands resulting from PCR-RFLP analysis. The cleavage products were analyzed on 2% agarose gel and visualized under UV light after staining with Ethidium bromide

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