Fig. 1
Detection of VDR gene FokI polymorphism. PCR amplification of the genomic DNA was carried out and the products of amplification were cleaved with restriction enzyme FokI (details given in Methods). Lane 1, phiX174 HaeIII cut Mr markers; lane 2, uncleaved PCR product; lane 3–5,7–9 & 13, PCR products from subjects having ff genotype; lane 6, pattern from a subject with homozygous FF genotype; lanes 10–12, pattern from subjects with heterozygous Ff genotype. The numbers on the side are sizes (bp) of the characteristic bands resulting from PCR-RFLP analysis. The restriction enzyme cleavage products were analyzed on 2% agarose gel and visualized under UV light after staining with Ethidium bromide