Fig. 1
From: Distribution of distinct subsets of circulating T follicular helper cells in Kawasaki disease
Flow cytometry analysis of the frequency of CD4+ T cells in KD patients. PBMCs from KD patients and control subjects were stained with fluorescent anti-CD3, anti-CD4, anti-CXCR5, anti-CD45RA-, anti-CXCR3 and anti-CCR6.The cells were gated initially on living lymphocytes, and then on CD3 + CD4+ T cells, and subsequently on CD45RA-CXCR5+ cTfh cells. The frequencies of CXCR3 + CCR6-, CXCR3-CCR6- and CXCR3-CCR6+ cTfh cell populations were analyzed by flow cytometry. a Flow cytometry analysis. b–h Quantitative analysis. Data shown are representative dot plots or are expressed as the percentage of cTfh cells of individual subjects. The horizontal lines represent the median values