Fig. 4

Immuno-localization of claudin proteins in control and NEC intestines. Representative images of intestines from 7-day control and NEC pups were dissected from the body and frozen in liquid nitrogen. Frozen sections were immunostained with anti-claudin-3, or -4, or -7 antibodies and detected by Cy3-conjugated secondary antibody. Nuclei were stained with DAPI (blue). The claudin signals were localized at cell-cell contact area as indicated by arrows in controls (CON). Arrows were pointed to the reduced signals in NEC samples and arrowheads in NEC showed the aggregated staining pattern. Scale bar: 50 μm