Exons | Primers | Sequence (5′-3′) | Annealing temperature (°C) |
---|---|---|---|
Exon1 | 1F | ATTTCCACTTCAGAAGCCTACT | 58 |
1R | GGCTCAAACAAACCATGGAAT | ||
Exon2 | 2F | GGGGTCAGAAGACAGAAGGAGGC | 64 |
2R | TGAGAACTGGCCCAAGTCGAGGA | ||
Exon3 | 3F | GTAATATCCGGGCTCAGTCACC | 64 |
3R | ACAGGTGGCACCTCCCGTCAG | ||
Exon4 | 4F | ATAGCTTGGCTTGAGGCCGTG | 62 |
4R | TTTGAAGGCAGAGTTCCTCTG | ||
Exon5–6 | 5–6F | CTGCTCTGAGCCTGTTTCC | 62 |
5–6R | ACGGTGCTTCCATCTTGAT | ||
Exon7 | 7F | CCGCCTTTCCATTGTTCC | 64 |
7R | CTCCCATCTGCCGCTGCACC | ||
Exon8 | 8F | AGGAAGGTTTCGGAGGGACT | 62 |
8R | TGAGACCAAGTCTGCAGTGCC | ||
Exon9 | 9F | GATGGACTGTCGGAGCGACACT | 64 |
9R | TCTTGGAGCTTGGGAACCGC | ||
Exon10 | 10F | AAGGGTCGACTGCGTGCAG | 64 |
10R | ATTCCAGCGAGAAAGGTG |