Fig. 2
From: High-dose of intravenous immunoglobulin modulates immune tolerance in premature infants
Representative gating scheme to identify regulatory and non-regulatory CD4+ T cells by flow cytometry. First, using the side scatter (SSC) and forward scatter (FCS) gate lymphocyte subgroup. Next, CD4+ T cells were based on the CD4/SSC strategy. Further sub-classified as CD4 + CD25 + Foxp3+, Tregs were identified by respective isotype control. a percentage of cell populations of the control group. b percentage of cell populations of the IVIG infusion group. Percentage of CD4 + T, CD4 + CD25+, and Foxp3+ Treg cells in the IVIG infusion group and the control (CTL) group. The percentage of CD4+ T (2c), CD4 + CD25+ (2d), and Foxp3+ Treg (2e) cells was measured with flow cytometry at times before and after IVIG infusion. Patients were grouped into IVIG infusion and control (CTL) groups. Means ± SD are shown