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Table 4 Reproducibility of the triplex qRT-PCR assay

From: Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms

  Target Conc. (copies/reaction) Number of determinations Mean Ct SD CV (%)
Intra-assay hMPV 108 3 13.90 0.033 0.24
102 3 35.69 0.170 0.47
RSV 108 3 15.80 0.015 0.10
102 3 39.10 0.112 0.29
Inter-assay hMPV 108 6 13.89 0.032 0.23
102 6 36.46 0.863 0.91
RSV 108 6 16.18 0.430 1.04
102 6 38.98 0.225 0.58
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