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Table 4 Reproducibility of the triplex qRT-PCR assay

From: Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms

 

Target

Conc. (copies/reaction)

Number of determinations

Mean Ct

SD

CV (%)

Intra-assay

hMPV

108

3

13.90

0.033

0.24

102

3

35.69

0.170

0.47

RSV

108

3

15.80

0.015

0.10

102

3

39.10

0.112

0.29

Inter-assay

hMPV

108

6

13.89

0.032

0.23

102

6

36.46

0.863

0.91

RSV

108

6

16.18

0.430

1.04

102

6

38.98

0.225

0.58