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Table 3 The linearity data for RSV and hMPV triplex qRT-PCR

From: Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms

Target

Slope

Intercept

r2

Linear range

(copies/reaction)

LODa

(copies/ reaction)

hMPV

−4.57

45.19

0.992

102–109

100

RSV

−3.91

44.85

0.998

102–109

100

  1. aLimit of detection