Fig. 2From: Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptomsCt values distribution of RSV and hMPV positive clinical specimens. Our one-step triplex qRT-PCR results showed that the viral load of the RSV or hMPV-positive samples from clinical specimens varied over a wide range, presenting threshold cycles between 21 and 44. RSV has a maximum occurrence detected between Ct 21 and 25, but hMPV has a maximum occurrence between Ct 31 and 35Back to article page