Skip to main content

Table 1 Serotype distribution among 193 S. pneumoniae isolates as determined by cpsB sequetyping and selected sequential mPCR

From: Using a practical molecular capsular serotype prediction strategy to investigate Streptococcus pneumoniae serotype distribution and antimicrobial resistance in Chinese local hospitalized children

Serotype/Serogroup

No. of isolates with serotypes determined by

Novel sequetypes

mPCR/6A-6D specific PCR

cpsB sequetypinga

3

3 (n = 2)

1 bp-3-2 (n = 2)

3-sz-1 (3–5)

6A

6/6A (n = 12)

2 bp-6C-6D-1 (n = 12)

6A-sz-1 (6A-5)

6B

6/6B (n = 13)

6B-1 (n = 1)

6B-6E-6X-1 (n = 12)

 

6C

6/6C (n = 2)

6C-6D-1 (n = 2)

 

9 V/9A

9 V/9A (n = 3)

9 V-1 (n = 3)

 

10B

unknown (n = 1)d

10B-1 (n = 1)

 

14

14 (n = 14)

14-1 (n = 14)

 

15 F/15A

15 F/15A (n = 2)

3 bp-15A-33B-1 (n = 2)b

15 F/15A-sz-1(15 F-15A-1)

15B/15C

15B/15C (n = 13)

15B-15C-1 (n = 13)

 

19 F

19 F (n = 67)

19 F-19A-1 (n = 67)

 

19A

19A (n = 23)

19A-2 (n = 23)

 

20

20 (n = 2)

13-20A-20B-1 (n = 2)b

 

23 F

23 F (n = 33)

23 F-1 (n = 20)

11 bp-11 F-1 (n = 9)c

6A-6B-6 F (n = 2)c

2 bp-6A-6B-6 F-1 (n = 1)c

13-20A-20B (n = 1)c

23 F-sz-1 (23 F-2)

23 F-6A-6B-6 F-sz-1

23 F-sz-2 (23 F-2)

23 F-13-20A-20B-1

23A

23A (n = 2)

23A-1 (n = 2)

 

28 F/28A

unknown (n = 1)d

28 F-28A-1 (n = 1)

 

34

34 (n = 1)

17A-34-1 (n = 1) b

 

unknown

unknown (n = 2)d

unknown (n = 2)

 
  1. a21 different sequetypes were identified
  2. bFive isolates with ambiguous sequetype result, mPCR confirmed them
  3. cThirteen 23 F isolates were of new sequetypes after mPCR was performed
  4. dFour isolates were untypeable by mPCR. Two isolates were identified by cpsB sequetyping. Two isolates were also unknown by sequencing