The panels on the left show representative photomicrographs of VEGF mRNA expression in the hypertrophic chondrocytes (7A), denoted by the black color and arrows, 50×; a
p < 0.03 Rapamycin (2 weeks) vs Control (2 weeks); b
p < 0.03 Rapamycin (4 weeks) vs Control (4 weeks). TRAP positive chondro/osteoclasts (7B), denoted by arrows and red color, 50×; ap < 0.01 Rapamycin (2 weeks) vs Control (2 weeks); bp < 0.03 Rapamycin (4 weeks) vs Control (4 weeks). The panels on the right show the quantification of the protein expression for VEGF (upper panel) and TRAP (lower panel) after 2 weeks and 4 weeks in Rapamycin and Control groups expressed as number of labeled cells to the total number of cells in the appropriate zone (Labeling Index).