Figure 5

The panels on the left show representative photomicrographs of p57 ^Kip2 protein expression in the hypertrophic chondrocytes (5A, denoted by arrows and brown color), 50×; ^a p < 0.05 Rapamycin (2 weeks) vs Control (2 weeks); ^b p < 0.05 Rapamycin (4 weeks) vs Control (4 weeks). p21 ^{Cip-1/SDI-1/WAF-1} protein expression localized only to the terminal hypertrophic chondrocytes (5B, denoted by arrows and brown color), 50×. The panels on the right show the quantification of the protein expression for p57 ^Kip2 (upper panel); and p21 ^{Cip-1/SDI-1/WAF-1} (lower panel) after 2 weeks and 4 weeks in Rapamycin and Control groups expressed as number of labeled cells to the total number of cells in the appropriate zone (Labeling Index).