Figure 3

The panels on the left show representative photomicrographs of PTH/PTHrP protein expression (3A) in the lower proliferating and upper hypertrophic chondrocytes, 50× (denoted by the purple color and arrows); ^a p < 0.004 Rapamycin (2 weeks) vs Rapamycin (4 weeks). Indian hedgehog protein expression in the lower proliferating and upper hypertrophic chondrocytes (3B, denoted by arrows and brown color), 50×; ^ap < 0.02 Rapamycin (2 weeks) vs Control (2 weeks); ^bp < 0.0001 Rapamycin (2 weeks) vs Rapamycin (4 weeks). The panels on the right show the quantification of the protein expression for PTH/PTHrP (upper panel); and Indian hedgehog (lower panel) after 2 weeks and 4 weeks in Rapamycin and Control groups expressed as number of labeled cells to the total number of cells in the appropriate zone (Labeling Index).