Figure 3

Indirect immunofluorescence of cryopreserved monkey cerebellum Sections of cryopreserved monkey cerebellum were reacted with human serum from a child with anti-centrosome antibodies and anti-pericentrin that was combined with low titer anti-Yo antibodies to visualize Purkinje cells. This shows that autoantibody reactivity is prominent in cells in the nuclear layer (left half of micrograph) and Purkinje cells (P, arrows) in the cerebellum and to a lesser extent in cells in the molecular layer (right half). Numerous discrete cytoplasmic speckles are seen in the Purkinje cells as illustrated in the enlarged cell (a, lower right inset). The nuclei of cells stained with DAPI (blue) is shown in panel b and this was overlayed on the IIF image from panel (a).