Table 3 Thermal profiles of RT-PCR
Specificity | Thermal profiles |
|---|---|
N. meningitides | 95°C for 2 min, followed by 50 cycles of 95°C for 5 sec and 60°C for 20 sec |
S. aureus | |
H. influenzae | |
S. pneumoniae | |
L.monocytogenes | 95°C for 2 min, followed by 50 cycles of 95°C for 5 sec and 63°C for 20 sec |
E.coli | 95°C for 30 sec, followed by 50 cycles of 95°C for 15 sec, 50°C for 30 sec and 75°C 20s |
S. agalactiae | 95°C for 30 sec, followed by 35 cycles of 94°C for 10 sec, 60°C for 15 sec and 72°C for 25s |