Table 2 Primers and probes used in RT-PCR assay
Specificity | Target | Primer and probe sequence (5′- 3′) | Application product (bp) | |
|---|---|---|---|---|
E.coli | 16S rRNA | F | GGGAGTAAAGTTAATACCTTTGC | 204 |
R | CTCAAGCTTGCCAGTATCAG | |||
Probe | FAM-CGCGATCACTCCGTGCCAGCAGCCGCGGATCGCG-BHQ1 | |||
L.monocytogenes | hly | F | CAT GGCACCACCAGC ATCT | 64 |
R | ATC CGCGTGTTTCTTTTCGA | |||
Probe | HEX-CGCCTG CAA GTC CTA AGA CGC CA-BHQ1 | |||
S. aureus | femA | F | TGCTGGTGGTACATCAAA | 97 |
R | ACGGTCAATGCCATGATTTAA | |||
Probe | FAM-ATTTTGCCGGAAGTTATGCAGTGCAATG-BHQ1 | |||
N. meningitidis | ctrA | F | TGTGTTCCGCTATACGCCATT | 114 |
R | GCCATATTCACACGATATACC | |||
Probe | FAM-AACCTTGAGCAA“T”CCATTTATCCTGACGTTCT-SpC6 “T”-BHQ1 | |||
H. influenzae | bexA | F | TGCGGTAGTGTTAGAAAATGGTATTATG | 116 |
R | GGACAAACATCACAAGCGGTTA | |||
Probe | HEX-ACAAAGCGTATCAA“T”ACTACAACGAGACGCAAAAA-SpC6 “T”-BHQ | |||
S. pneumoniae | lytA | F | ACGCAATCTAGCAGATGAAGCA | 75 |
R | TCGTGCGTTTTAATTCCAGCT | |||
Probe | FAM-TGCCGAAAACGCTTGATACAGGGAG-BHQ1 | |||
S. agalactiae | cfb | F | CGCAATGAAGTCTTTAATTTTTC | 260 |
R | ATGATGTATCTATCTGGAACTCTAGTG |